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目的对幽门螺杆菌(Helicobacter pylori,Hp)感染蒙古沙鼠模型胃黏膜菌群多样性进行研究,探讨Hp感染与胃内菌群的关系。方法建立Hp感染的蒙古沙鼠模型,空白对照组、阴性对照组、直接感染组和预处理感染组各15例,4周后采集胃黏膜样本,同时检测HP定植率,并提取细菌基因组DNA,采用聚合酶链反应-变性梯度凝胶电泳技术(PCR-DGGE)对黏膜局部菌群进行指纹图谱分析,并对特异性条带进行测序鉴定分析。结果预处理感染组感染率(93.3%)与直接感染组感染率(26.7%)有明显差异(P<0.05);各实验组PCR-DGGE指纹图谱分析显示条带数量,空白对照组(21.6±2.5)、阴性对照组(3.3±1.1)、直接感染组(14.6±2.4)和预处理感染组(7.2±2.2),经统计学分析,各组间均有明显差异(P<0.05),提示蒙古沙鼠各组间菌群多样性存在显著的差异性。结论 Hp感染与胃黏膜菌群结构的变化密切相关。
Objective To study the diversity of gastric mucosa in Helicobacter pylori (Hp) infected Mongolian gerbil and explore the relationship between Hp infection and the intragastric flora. Methods The H. pylori infection model of Mongolian gerbils was established. Fifteen cases were divided into blank control group, negative control group, direct infection group and preconditioning infection group. Gastric mucosa samples were collected after 4 weeks. HP colonization rate was also detected. Bacterial genomic DNA was extracted, Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze the microflora of the mucosa. The specific bands were identified by sequencing. Results The infection rate of pretreatment group (93.3%) was significantly different from that of direct infection group (26.7%) (P <0.05). Fingerprint analysis of PCR-DGGE in each experimental group showed that the number of bands, 2.5), negative control group (3.3 ± 1.1), direct infection group (14.6 ± 2.4) and pretreatment infection group (7.2 ± 2.2). There were significant differences among groups (P <0.05) Mongolian gerbils flora diversity between groups there are significant differences. Conclusion Hp infection is closely related to the changes of gastric mucosal flora.