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目的了解中国汉族群体与德国群体人类ｃ－ｆｅｓ／ｆｐｓ原癌基因座（ＦＥＳ）的遗传多态性，获得中国汉族和德国群体ＦＥＳ基因座的群体遗传数据。方法ＥＤＴＡ抗凝血样采自３１１名无血缘关系的中国汉族个体和１２３名无血缘关系的德国个体。Ｃｈｅｌｅｘ法提取ＤＮＡ。ＰＣＲ扩增样本ＤＮＡ，聚丙烯酰胺凝胶水平电泳分型，自动激光荧光测序仪测定ＤＮＡ序列。结果在中国汉族群体中ＦＥＳ基因座存在９个等位基因，等位基因频率均以ＦＥＳ＊１１为最高，ＦＥＳ＊１２和ＦＥＳ＊１３较高。发现德国群体中ＦＥＳ基因座５′端第３４碱基存在Ｃ替换Ａ的变异。共存在９个等位基因，等位基因频率以ＦＥＳ＊１１为最高，ＦＥＳ＊１０ａ和ＦＥＳ＊１２较高。中国汉族群体和德国群体基因型分布均符合Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡。结论３个汉族群体之间比较基因频率无显著性差异。３个中国汉族群体与德国群体比较基因频率差异显著。
Objective To understand the genetic polymorphisms of c-fes / fps protooncogenes (FES) in Chinese Han population and German population and to obtain population genetic data of FES loci in Chinese Han and German populations. Methods EDTA anticoagulant blood samples were collected from 311 unrelated Chinese Han individuals and 123 unrelated German individuals. Chelex method to extract DNA. PCR amplification of sample DNA, polyacrylamide gel electrophoresis typing, automated laser fluorescence sequencer DNA sequence. Results There were 9 alleles in FES locus in Chinese Han population. The allele frequency was highest in FES * 11 and higher in FES * 12 and FES * 13. It was found that there was a C substitution A variation in the 34th base of the 5 ’end of the FES locus in the German population. There were 9 alleles in total, allele frequencies were highest in FES * 11 and higher in FES * 10a and FES * 12. The distribution of genotypes in Chinese Han population and German population all fit Hardy-Weinberg equilibrium. Conclusion There is no significant difference in the frequency of comparative genes between the three Han populations. There was significant difference in the frequency of comparative genes between the three Chinese Han nationality groups and the German one.