论文部分内容阅读
目的检测慢性胃炎儿童胃液中幽门螺杆菌DNA，探讨其在临床诊断中的实用价值。方法应用PCR检测胃液中幽门螺旋菌DNA，应用Warthin-Starry银染色检测胃粘膜组织中的幽门螺旋菌。结果胃炎组与非胃炎组比较，敏感性为75.0％，特异性为96．2％，两组有显著性差异（P＜0．01）。PCR与Warthin－Starry银染色比较，PCR优于Warthin—Starry银染色法。结论PCR检测患儿胃液中的幽门螺旋菌DNA，具有敏感、快速、特异、高效等优点，是基因水平上检测幽门螺旋菌一项新技术，用于慢性胃炎的早期诊断，具有较好的实用价值，值得推广应用。
Objective To detect Helicobacter pylori DNA in gastric juice of children with chronic gastritis and to explore its practical value in clinical diagnosis. Methods Helicobacter pylori DNA in gastric juice was detected by PCR. Helicobacter pylori in gastric mucosa was detected by Warthin-Starry silver staining. Results The sensitivity and the specificity of gastritis group were 75.0% and 96.2%, respectively, which were significantly different between the two groups (P <0.01). PCR was superior to Warthin-Starry silver staining when compared to Warthin-Starry silver staining. Conclusion PCR detection of Helicobacter pylori DNA in gastric juice is sensitive, rapid, specific and efficient. It is a new technique for detecting Helicobacter pylori at the gene level for the early diagnosis of chronic gastritis and has good practicality Value, it is worth promoting application.