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目的 观察芹灵冲剂（Ｑｉｎｌｉｎｇ ｃｈｏｎｇｊｉ， ＱＬ） 对ＤＨＢＶ 肝炎雏鸭肝脏的保护作用及对肝炎雏鸭肾功能、血液生化改变的影响．方法 待ＤＨＢＶ 感染雏鸭７ ｄ 血清检测为阳性后，随机分组进行药物治疗试验，每组５ ～６ 只，ＱＬ 分为高（ＱＬｂ） 、中（ＱＬｍ） 、低（ＱＬｓ）３ 个剂量组，分别为１０ ，５ 和２－５ ｇ／ ｋｇ 组，ｉｇ ，２ 次／ ｄ ，１０ ｄ ． 设病毒对照组（ＤＨＢＶ） ，以生理盐水代替药物． 阳性药用阿昔洛韦（ 无环鸟苷，ＡＣＶ） 粉剂１００ ｍｇ／ ｋｇ ，ｉｇ ．２ 次／ ｄ，１０ ｄ ，自鸭腿胫静脉取血，分离血清，用全自动生化测定仪分别测定肝功能（ＡＬＴ，ＡＳＴ，ＳＢ） 、肾功能（ ＵＮ，ＣＲ） 及其他生化指标的含量． 并将各组动物采血后剖腹取出肝脏，在肝脏同一部位取样，１００ ｍ Ｌ／ Ｌ中性福尔马林液固定，常规石蜡包埋切片，ＨＥ 染色，光镜观察．结果 雏鸭被ＤＨＢＶ 感染７ ｄ 后血液生化指标明显异常，其中肝功能（ＡＬＴ，ＡＳＴ，ＳＢ） 和肾功能（ＵＮ，ＣＲ） 改变最明显，其次乳酸脱氢酶（ＬＤＨ） 显著升高． 用药组经不同浓度的ＱＬ 分别治疗１０ ｄ 后，尤其ＱＬｂ（１０ ｇ／ ｋｇ） 对上述异常升高的血液生化指标均显?
Objective To observe the protective effect of Qinling chongji (QL) on the liver of ducklings infected with DHBV and the effect on the renal function and blood biochemical changes of hepatitis ducklings. Methods After the ducklings infected with DHBV were tested positive for 7 days, they were randomly divided into groups of 5 to 6 drugs. QL was divided into 3 groups: high (QLb), medium (QLm), and low (QLs). 10, 5, and 2-5 g/kg groups, ig, 2 times/d, 10 d, respectively. Let the virus control group (DHBV) replace saline with saline. Positive drug aciclovir (acyclovir ACV) powder 100 mg/kg, ig. 2 times / d, 10 days, blood was collected from duck leg veins, serum was separated, and the contents of liver function (ALT, AST, SB), renal function (UN, CR) and other biochemical indexes were measured by an automatic biochemical analyzer. . After taking blood from each group of animals, the liver was removed by laparotomy and the liver was sampled in the same part of the liver. The 100 m L/L neutral formalin solution was fixed and paraffin-embedded and routinely stained with HE and observed by light microscopy. Results The blood biochemical parameters of ducklings were significantly abnormal after being infected by DHBV for 7 days. Among them, liver function (ALT, AST, SB) and renal function (UN, CR) changed most significantly, followed by lactate dehydrogenase (LDH). After the treatment group was treated with different concentration of QL for 10 days, especially QLb (10 g/kg) showed abnormally elevated blood biochemical indicators.