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目的为了研究不同肝病患者人群中红细胞ｃｒ１基因组密度多态性与红细胞免疫粘附肿瘤细胞能力的相关性．方法乙型肝炎患者４８例，肝硬变４９例，肝细胞性肝癌９４例及正常对照８０例．采用ＰＣＲ加ＨｉｎｄⅢ酶切技术测定红细胞ｃｒ１基因组密度多态型（ＨＨ型、ＨＬ型、ＬＬ型），采用肿瘤红细胞花环试验测定红细胞免疫粘附肿瘤细胞的能力．结果在不同人群中，ＨＨ型红细胞免疫粘附肿瘤细胞的能力明显大于ＨＬ型红细胞，而ＨＬ型红细胞免疫粘附肿瘤细胞的能力明显大于ＬＬ型红细胞．在相同基因型人群中，正常人红细胞免疫粘附肿瘤细胞能力显著高于乙型肝炎、肝硬变和肝癌患者．结论红细胞免疫粘附肿瘤细胞的能力与ｃｒ１基因组密度多态型别有关，同时也与后天获得性影响因素有关．
Objective To investigate the association of erythrocyte cr1 genomic density polymorphism with erythrocyte immune adherence to tumor cells in populations with different liver diseases. Methods 48 patients with hepatitis B, cirrhosis in 49 cases, hepatocellular carcinoma in 94 cases and 80 cases of normal control. The density of erythrocytes cr1 genomic polymorphism (HH, HL and LL) was determined by PCR and Hind Ⅲ digestion. The ability of erythrocytes to adhere to tumor cells was determined by the tumor erythrocyte rosette assay. Results In different populations, the ability of HH-type erythrocytes to adhere to tumor cells was significantly greater than that of HL-type erythrocytes. However, the ability of HL-type erythrocytes to adhere to tumor cells was significantly greater than that of LL-type erythrocytes. In the same genotype population, the normal human erythrocyte immune adhesion tumor cells were significantly higher than the ability of hepatitis B, cirrhosis and liver cancer patients. Conclusion The ability of erythrocytes to adhere to tumor cells is related to the polymorphism of cr1 genomic density and to the acquired factors.