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目的:探讨蝎毒多肽对白血病细胞株KG1a干细胞活性的影响。方法:免疫磁珠法分离出CD34+CD38-的KG1a细胞,按照不同给药方式分空白组、PESV组、DNR组、PESV+DNR组,培养后经WST-8、流式细胞仪分别检测KG1a干细胞增殖抑制率、细胞凋亡率和细胞周期,并经RT-PCR检测PTEN基因、Tie-2基因mRNA表达。结果:KG1a干细胞在PESV组、DNR组细胞增殖抑制率(%)与空白组相比差异有统计学意义(P<0.05)。诱导细胞凋亡作用强弱:PESV+DNR组>DNR组>PESV组。细胞周期检测结果显示,G0/G1期PESV+DNR组与PESV组、DNR组相比差异有统计学意义(P<0.01)。在G2/M期PESV+DNR组与PESV组、DNR组相比差异有统计学意义(P<0.05)。PESV组、DNR组、PESV+DNR组KG1a干细胞的PTEN基因、tie-2基因表达均上调,PESV+DNR组为高表达。结论:PESV能够抑制KG1a干细胞增殖,但不能增强DNR的抑制作用;PESV对DNR损伤KG1a干细胞有诱导凋亡和分化的作用,其机制可能与上调KG1a干细胞的PTEN基因、tie-2基因表达有关。
Objective: To investigate the effect of scorpion venom polypeptide on the activity of leukemia cell line KG1a. Methods: KG1a cells isolated from CD34 + CD38- cells by immunomagnetic beads method were divided into blank group, PESV group, DNR group and PESV + DNR group according to different administration methods. After cultured, the expression of KG1a was detected by WST-8 and flow cytometry Inhibition rate of stem cell proliferation, apoptosis rate and cell cycle. The mRNA expressions of PTEN and Tie-2 were detected by RT-PCR. Results: The inhibition rate (%) of KG1a stem cells in PESV group and DNR group was significantly lower than that in blank group (P <0.05). The effect of inducing apoptosis: PESV + DNR group> DNR group> PESV group. Cell cycle analysis showed that there was significant difference between PESV + DNR group and PESV group and DNR group at G0 / G1 phase (P <0.01). There was significant difference between PESV + DNR group and PESV group and DNR group in G2 / M phase (P <0.05). PTEN gene and tie-2 gene expression were up-regulated in PESV group, DNR group and PESV + DNR group KG1a stem cells, PESV + DNR group was high expression. CONCLUSION: PESV can inhibit the proliferation of KG1a stem cells but not enhance the inhibition of DNR. PESV can induce the apoptosis and differentiation of KG1a stem cells induced by DNR. The mechanism may be related to the up-regulation of PTEN gene and tie-2 gene expression in KG1a stem cells.