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为研究胃癌中P16基因在蛋白水平的表达,应用蛋白A金探针免疫组化技术对30例胃癌和20例正常胃粘膜标本P16进行分析。结果所有正常对照组均有P16表达,其阳性细胞百分数为72.55％±17.22％,而胃癌组为54.90％±28.90％,明显低于正常对照(t=2.100,P<0.05)。胃痛组中23.3％(7/30)P16表达减低,10％(3/30)未表达。提示胃癌中有P16的异常表达,免疫组化技术可较准确地反映P16的表达,更适合于临床研究。
In order to study the expression of P16 gene in gastric cancer, protein A gold probe immunohistochemical technique was used to analyze 30 cases of gastric cancer and 20 cases of normal gastric mucosa specimens P16. Results The expression of P16 in all normal control groups was 72.55%±17.22%, while that in gastric cancer was 54.90%±28.90%, which was significantly lower than that of normal controls (t=2.100, P<0.05). The expression of P16 in 23.3% (7/30) of the stomach pain group was reduced, and 10% (3/30) was not expressed. It is suggested that there is abnormal expression of P16 in gastric cancer. Immunohistochemistry can accurately reflect the expression of P16 and is more suitable for clinical research.