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通过组织培养建立乌菜离体再生体系,可使优良珍贵育种材料得到保存,并能为其快速繁殖提供有效途径。本研究以4种不同基因型乌菜为材料,从外植体类型、基本培养基类型、植物生长调节剂配比、基因型及无菌苗苗龄等方面,对影响乌菜不定芽诱导的因素进行了研究。研究表明,7-1基因型乌菜5 d苗龄的带柄子叶外植体,在以MS为基本培养基(3%蔗糖+0.8%琼脂)添加4 mg/L 6-BA与0.5 mg/Lα-NAA的诱导培养基上进行诱导,不定芽诱导率可达85.56%。本研究对影响乌菜植株再生的多个因素进行了优化,建立了高效离体再生技术体系,为乌菜育种研究及其他生物技术研究提供基础。
Through the tissue culture to establish the regeneration system of the wild cabbage, the valuable precious breeding material can be preserved, and can provide an effective way for its rapid propagation. In this study, four different genotypes of Radix et Rhizoma were used as materials to study the effects of different types of shoots on the induction of adventitious buds from the explants, the type of basic medium, the ratio of plant growth regulators, the genotype and the seedling age of sterile germ. Factors have been studied. The results showed that the explants with cotyledons of 5-day-old seedlings with 7-1 genotypes had the best performance in the medium with MS as the basic medium (3% sucrose + 0.8% agar) with 4 mg / L 6-BA and 0.5 mg / Lα-NAA induction medium induction, adventitious bud induction rate of up to 85.56%. In this study, a number of factors influencing the regeneration of the plant were optimized, and a highly efficient system for in vitro regeneration was established, which provided the basis for the research on the breeding and other biotechnology of the plant.