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目的建立曲马多依赖大鼠动物模型。方法 30只大鼠平均分为3组,观察以60mg/kg曲马多灌胃给药与停药戒断过程中大鼠的身体依赖和精神依赖表现。结果曲马多依赖组大鼠在给药后15min内,对声音及触摸刺激反应性增高,奔跑、跳跃、竖尾等现象为3.9±0.4次/只,明显高于对照组的0.5±0.1次/只(P<0.05);条件性位置偏爱实验中,曲马多依赖组大鼠明显偏爱给药的白箱(P<0.05)。而戒断组在停止给药后出现烦躁不安、高度激惹、尖叫等行为4.9±0.7次/只,高于依赖组的0.6±0.2次/只(P<0.05);依赖组与戒断组大鼠体重明显下降(P<0.05)。结论以60mg/kg灌胃给药112d,大鼠对曲马多产生依赖,曲马多依赖大鼠动物模型建立成功。
Objective To establish a rat model of tramadol dependence. Methods Thirty rats were equally divided into three groups. The body dependence and mental dependence of rats during tramadol 60 mg / kg intragastric administration and withdrawal were observed. Results The tramadol-dependent rats responded to the sound and touch stimuli within 15 minutes after administration, with the mean value of 3.9 ± 0.4 times per run, jumping, and vertical tail significantly higher than that of the control group of 0.5 ± 0.1 times / Only (P <0.05). In the conditioned place preference test, the tramadol dependence group obviously favored the administration of white box (P <0.05). The abstinence group showed no irritability, high irritability, screaming and other behaviors 4.9 ± 0.7 times / only after stopping administration, higher than the 0.6 ± 0.2 times / dependency group (P <0.05) The body weight of rats decreased significantly (P <0.05). Conclusion Administration of 60mg / kg intragastrically for 112 days results in a dependence of tramadol in rats, and a successful tramadol-dependent rat model.