目的　为证明甲基莲心碱（ Ｎｅｆｅｒｉｎｅ， Ｎｅｆ） 对单个心肌细胞离子通道电流的影响及抗心律失常作用的离子通道机制。方法　采用全细胞记录膜片钳技术，记录了 Ｎｅｆ 对培养大鼠心室肌细胞钠电流（ Ｉ Ｎａ） 及豚鼠心室肌细胞动作电位、 Ｉ Ｎａ 、 Ｌ 型钙电流（ Ｉ Ｃａ Ｌ） 及稳态外向 Ｋ＋ 电流的影响。结果　 Ｎｅｆ ３０ ，１００ μｍｏｌ· Ｌ－ １ 明显抑制培养大鼠心室肌细胞 Ｉ Ｎａ ，分别从对照水平的（３４ ±０７） ｎ Ａ 降至（２１ ±０５） 和（０４ ±０２） ｎ Ａ。 Ｎｅｆ １０ μｍｏｌ· Ｌ－ １ 可降低豚鼠心室肌细胞动作电位振幅、静息电位，延长动作电位时程。 Ｎｅｆ １０ ，３０ μｍｏｌ· Ｌ－ １ 分别使豚鼠心室肌细胞 Ｉ Ｎａ 及 Ｉ Ｃａ Ｌ从给药前的（７９ ±２１） ｎ Ａ 和（６８９ ±２４３） ｐ Ａ 降至（４０ ±１４） 、（１３ ±０６） ｎ Ａ和（３７４ ±１７２） 、（１０９ ±６７） ｐ Ａ。 Ｎｅｆ １０ μｍｏｌ· Ｌ－ １ 还抑制 Ｉ Ｎａ 、稳态外向 Ｋ＋ 电流和 Ｉ Ｃａ Ｌ的 Ｉ Ｖ 曲线并使后者的峰值电流电位略右移。结论　 Ｎｅｆ 有钠、 Ｌ 型钙通道阻滞作用并抑制稳态外向 Ｋ＋ 电流，这些可解释 Objective To demonstrate the influence of Neferine (Nef) on the ion channel currents of single cardiomyocytes and the ion channel mechanism of antiarrhythmic effect. Methods The whole-cell recording patch-clamp technique was used to record the effects of Nef on the sodium currents (I Na) and the action potentials of I Na and L-type calcium currents (I Ca-L) and steady state Outward K + current effects. Results Nef 30, 100 μmol · L -1 significantly inhibited I Na in cultured rat ventricular myocytes, from (34 ± 07) n A to (21 ± 05) and 0  4 ± 0  2) n A. Nef 10 μmol · L -1 can reduce the amplitude of action potential and resting potential of guinea pig ventricular myocytes and prolong the action potential duration. Nef 10 and 30 μmol · L -1 decreased I Na and I CaL in guinea pig ventricular myocytes from (79 ± 21) n A and (689 ± 243) p A to (4  ± 14), (13 ± 06) n A and (374 ± 172), (109 ± 67) p A Nef 10 μmol · L -1 also inhibited the I Na, steady-state outward K + current and I V-V curve of I Ca-L and slightly shifted the latter peak current potential to the right. Conclusion Nef has sodium and L-type calcium channel blockade and inhibits steady-state outward K + current, which can explain