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巨噬细胞集落刺激因子 (M CSF )是一种单核吞噬细胞特异性的生长因子。近年来的研究表明 ,M CSF可以增强巨噬细胞杀伤细菌等病原体及肿瘤细胞的能力 ,对抗感染及抗肿瘤具有重要意义。一氧化氮 (NO )是可由单核吞噬细胞产生的一种自由基 ,对病原体及肿瘤细胞同样具有杀伤作用。为探讨M CSF的抗感染、肿瘤作用是否与其刺激单核吞噬细胞产生NO有关 ,我们以L92 9细胞条件培养液 (L92 9 CM )作为M CSF来源 ,观察了M CSF对巨噬细胞系RAW2 64 7细胞NO产生的影响。结果发现 ,L92 9 CM可以刺激RAW2 64 7细胞NO的产生 ;且RT PCR显示L92 9 CM处理使RAW2 64 7细胞诱导型NO合酶 (iNOS )表达增加。
Macrophage colony-stimulating factor (M CSF) is a mononuclear phagocyte-specific growth factor. In recent years, studies have shown that M CSF can enhance the ability of macrophages to kill pathogens such as bacteria and tumor cells, and is of great significance in fighting infection and anti-tumor. Nitric oxide (NO) is a free radical that can be produced by mononuclear phagocytes and has the same killer effect on pathogens and tumor cells. In order to investigate whether anti-M-M is anti-infective, and whether tumor function is related to stimulating NO production by mononuclear phagocytes, L92 9 cells conditioned medium (L92 9 CM) was used as the source of M CSF. The effect of M CSF on macrophage cell line RAW264 7 cells NO production. The results showed that L92 9 CM stimulated NO production in RAW2647 cells, and RT-PCR showed that L92 9 CM increased the expression of inducible NO synthase (iNOS) in RAW2647 cells.