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目的探讨新生大鼠心、肝、脾、肺、肾和皮肤中成纤维细胞nanog蛋白表达差异及其意义。方法用免疫组织化学、免疫荧光和体外细胞培养技术,对成纤维细胞进行nanog蛋白和波形蛋白(vimentin)标记,检测并比较新生大鼠不同器官中成纤维细胞nanog蛋白和vimentin表达及共表达特征。结果新生大鼠心、肝、脾、肺、肾和皮肤6种器官中均有nanog蛋白和vimentin表达,在每个器官中均有少数成纤维细胞共表达这两种蛋白,共表达率分别为心(10.07±0.77)%、肝(9.97±3.70)%、脾(11.23±2.98)%、肺(15.07±2.87)%、肾(11.75±2.76)%、皮肤(16.14±1.28)%,其中皮肤和肺的共表达率高于心、肝、脾、肾(P<0.05)。结论共表达nanog蛋白和vimentin的成纤维细胞可能更具有干细胞样特性;新生大鼠皮肤和肺的成纤维细胞可能更具有组织损伤修复潜能。
Objective To investigate the difference and significance of nanog protein expression in fibroblasts in heart, liver, spleen, lung, kidney and skin of newborn rats. Methods The fibroblasts were labeled with nanog protein and vimentin by immunohistochemistry, immunofluorescence and in vitro cell culture. The expression and co-expression of nanog protein and vimentin in fibroblasts from different organs of neonatal rats were detected and compared . Results Nanog protein and vimentin were expressed in 6 kinds of organs of heart, liver, spleen, lung, kidney and skin of neonatal rats. A small number of fibroblasts in each organ co-expressed these two proteins, Heart (10.07 ± 0.77)%, liver (9.97 ± 3.70)%, spleen (11.23 ± 2.98)%, lung (15.07 ± 2.87)%, kidney (11.75 ± 2.76)% and skin And lung co-expression rate higher than the heart, liver, spleen, kidney (P <0.05). Conclusions Fibroblasts that co-express nanog protein and vimentin may be more stem-like; the fibroblasts of neonatal rat skin and lung may be more likely to have tissue repair potential.