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随着淋巴细胞研究的深入,越来越需要建立检测细胞表面抗原的可靠敏感而又简便的方法。现用的直接膜免疫荧光技术(DIF)不仅在检测各种淋巴细胞标志中占有重要地位,而且促进了荧光激活细胞分离器(FACS)的发展。但是,DIF也有缺点,如荧光着色弱、易退色、需要专门的荧光设备和技术、受细胞IgG Fc(Fcγ)受体和外源性Ig的影响等等。近几年来,一些学者采用了一种新的抗球蛋白花环反应技术,证明是一种特异性良好、敏感性高的简便方法。但也和DIF一样,需要经验和认真操作,方能获得可靠结果。本文以检测淋巴细胞SIg为重点,介绍抗球蛋白花环反应的研究进展。 一、抗球蛋白花环反应的类型和原理
As lymphocyte research progresses, there is an increasing need to establish a reliable, sensitive, and simple method of detecting cell surface antigens. Current direct membrane immunofluorescence (DIF) not only plays an important role in the detection of various lymphocyte markers, but also promotes the development of fluorescence activated cell separators (FACS). However, DIF also has disadvantages such as weak fluorescence coloration, easy fading, special fluorescence equipment and techniques, influence by cellular IgG Fc (Fcγ) receptors and exogenous Ig, and the like. In recent years, some scholars have adopted a new antiglobulin garland reaction technology, which proves to be a convenient and sensitive method with high specificity. But like the DIF, it requires experience and serious action to get reliable results. In this paper, we will focus on the detection of lymphocyte SIg and introduce the research progress of anti-globulin garland. First, anti-globulin garland reaction type and principle