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[目的]探讨3-甲基腺嘌呤(3-MA)对染矽尘大鼠肺组织胶原纤维m RNA表达的影响。[方法]80只健康雄性大鼠建立矽肺模型,随机分为对照组和3-MA组,3-MA组腹腔注射1.5 mg/(kg·d)3-MA,隔天给药持续28 d,染尘不同时间处死,HE染色观察大鼠肺组织病理组织学变化,测定肺组织中羟脯氨酸、Ⅰ型和Ⅲ型胶原纤维m RNA含量。[结果]HE染色显示,3-MA组每个时间点肺组织纤维化程度均比对照组轻,随着染尘时间延长肺组织纤维化有不同程度的改变。3-MA组大鼠7 d(0.39±0.02)、14 d(0.43±0.01)、28 d(0.36±0.02)、60 d(0.34±0.04)、90 d(0.49±0.03)肺组织羟脯氨酸含量均低于同一时间点对照组大鼠(0.41±0.03,0.48±0.01,0.49±0.02,0.60±03.02,0.73±0.03)(P<0.05),不同时间点羟脯氨酸含量差异有统计学意义(F=55.99,P<0.05)。3-MA组7 d肺组织Ⅰ型胶原纤维m RNA含量(1.11±0.30)比对照组(0.83±0.13)高,除28 d外其余各时间点(14 d:0.70±0.23,60 d:0.92±0.08,90 d:1.38±0.37)均低于对照组(14 d:1.06±0.16,60 d:1.10±0.25,90 d:2.06±0.66)(P<0.05),不同时间点其差异有统计学意义(H=23.36,P<0.05)。3-MA组7 d肺组织Ⅲ型胶原m RNA含量较高(0.77±0.19),在14 d(0.44±0.26)、28 d(0.35±0.09)及60 d(0.31±0.06)时呈现下降趋势,90 d(1.44±0.31)时又再度升高,各时间点差异有统计学意义(H=28.77,P<0.05),各时间点其含量均低于对照组(7 d:0.83±0.13,14 d:1.06±0.16,28 d:0.98±0.11,60 d:1.10±0.25,90 d:2.06±0.67)(P<0.05)。[结论]3-MA干预后,与对照组相比同一时间点及同一组不同时间大鼠肺组织胶原纤维m RNA表达不同,影响大鼠矽肺纤维化发生发展进程。
[Objective] To investigate the effect of 3-methyladenine (3-MA) on the expression of collagen mRNA in the lung of rats exposed to silica dust. [Methods] Silicone lung model was established in 80 healthy male rats and randomly divided into control group and 3-MA group. The 3-MA group was given intraperitoneal injection of 3-MA 1.5 mg / (kg · d) Dysplasia was sacrificed at different times and the histopathological changes of lung tissue were observed by HE staining. The contents of hydroxyproline, type I and type III collagen fibers m RNA in lung tissue were determined. [Result] The results of HE staining showed that the degree of lung fibrosis in 3-MA group was lighter than that in control group at each time point, and the degree of lung fibrosis changed with the prolongation of dusting time. In the 3-MA group, the level of hydroxyproline in the lung tissue was significantly higher in the 7th day (0.39 ± 0.02), 14th day (0.43 ± 0.01), 28th day (0.36 ± 0.02), 60th day (0.34 ± 0.04), 90th day (P <0.05). The contents of hydroxyproline in different time points were statistically different Significance (F = 55.99, P <0.05). The mRNA expression of type I collagen mRNA in lung tissue of 3-MA group (1.11 ± 0.30) was higher than that of control group (0.83 ± 0.13) on day 7, except the 28th day ± 0.08,90 d: 1.38 ± 0.37) were lower than the control group (14 d: 1.06 ± 0.16,60 d: 1.10 ± 0.25,90 d: 2.06 ± 0.66) (P <0.05) Significance (H = 23.36, P <0.05). The level of m RNA in type Ⅲ collagen of 3-MA group was higher at 7 d (0.77 ± 0.19), showing a decreasing trend at 14 d (0.44 ± 0.26), 28 d (0.35 ± 0.09) and 60 d (0.31 ± 0.06) , And increased again at 90 d (1.44 ± 0.31), with statistical significance at each time point (H = 28.77, P <0.05), and the contents were lower at each time point than those in the control group (7 d: 0.83 ± 0.13, 14 d: 1.06 ± 0.16, 28 d: 0.98 ± 0.11, 60 d: 1.10 ± 0.25, 90 d: 2.06 ± 0.67) (P <0.05). [Conclusion] Compared with the control group, the expression of m RNA in the lung tissue of rats at the same time point and in the same group at different time after 3-MA intervention were different, which affected the occurrence and development of silicotic fibrosis in rats.