作者选用1株高特异性抗旋毛虫单克隆抗体2G_8B_(10)H_2,建立了单克隆抗体竞争酶联免疫吸附试验(competitive ELISA,简称cELISA),并进行了初步应用。结果表明:20只日本大耳兔在感染旋毛虫后17天有35％呈现阳性,31天全部阳性.对其中5只感染兔的血清终点滴度观察了142天,了解特异性抗体的变化规律.该规律为病程估计提供了线索。另外,其他蠕虫感染兔血清29份无1例假阳性发生。可以认为,单克隆抗体cELISA是一种灵敏、特异的旋毛虫病免疫诊断方法。 The authors selected a highly specific anti-Trichinella monoclonal antibody 2G_8B_ (10) H_2 monoclonal antibody competitive ELISA test (competitive ELISA, referred to as cELISA), and the initial application. The results showed that 35% of 20 Japanese rabbits were positive 17 days after infection with Trichinella spiralis and all were positive on day 31. Serum final titers of 5 infected rabbits were observed for 142 days to understand the variation of specific antibodies This rule provides clues to the course estimation. In addition, none of the 29 other rabbits infected with rabbit serum detected false positives. It can be considered, monoclonal antibody cELISA is a sensitive and specific method of immune diagnosis of Trichinellosis.