论文部分内容阅读
降低血型抗原的异体免疫水平对于慢性输血病人有很重要的临床意义.利用甲氧基聚乙二醇修饰红细胞的策略,可以免疫修饰血型抗原以降低红细胞的异体免疫水平.本研究显示,抗体识别非ABO的水平随甲氧基聚乙二醇的分子大小和浓度的升高而显著下降,分子量大的多聚体的免疫保护作用比分子量小的显著.同时,动物实验表明,甲氧基聚乙二醇修饰的异种红细胞(羊红细胞)以及同种不同品系(C57Bl/6)或自体(Balb/c)红细胞的体内免疫原性均明显降低.与对照相比,小鼠接受甲氧基聚乙二醇修饰的绵羊血,发现抗绵羊红细胞的抗体产生降低了90%.与对照相比,两次输入甲氧基聚乙二醇修饰的绵羊血的小鼠,抗绵羊血抗体产生降低了80%.尤为重要的是,甲氧基聚乙二醇修饰的自体血细胞没有诱导新抗原的产生,也没有IgG或IgM应答.以上结果表明,聚合物对红细胞的免疫修饰可以安全有效地降低异体免疫的水平,具有潜在的输血医学临床应用价值.
Reducing the level of allogeneic immunity of blood group antigen is very important clinical significance for patients with chronic blood transfusion.Using methoxypolyethylene glycol to modify erythrocytes can immunologically modify blood group antigen to reduce the level of allogeneic immunity of erythrocytes.This study shows that antibody recognition The level of non-ABO decreased significantly with the increase of the molecular size and concentration of methoxypolyethylene glycol, while the protection of high molecular weight multimers was significantly less than the molecular weight.At the same time, animal experiments showed that the methoxyl poly Glycol-modified xenogeneic erythrocytes (sheep erythrocytes) as well as in vivo immunogenicity of the same strain (C57BI / 6) or autologous (Balb / c) erythrocytes were significantly reduced.Compared with the control mice received methoxy poly Ethylene glycol modified sheep blood and a 90% reduction in antibody production against sheep red blood cells was found.Compared with the control, mice raised with methoxy polyethylene glycol-modified sheep blood twice reduced anti-sheep blood antibody production 80% .More importantly, methoxy polyethylene glycol-modified autologous blood cells did not induce the production of new antigens, nor IgG or IgM response.The above results show that the polymer can be immune immune modification of red blood cells Effectively reduce the level of allogeneic immune, blood transfusion has potential application of clinical medicine.