肿瘤特异性抗原(TSA)或其抗独特型抗体已被用于控制动物模型中肿瘤的生长,但有效的免疫应答需要抗原和宿主组织相容性决定簇的同时提呈。作者用表达多瘤病毒(PY)蛋白的牛痘重组株接种动物模型来了解在宿主细胞中表达TSA的活的牛痘重组病毒能否更好地激发肿瘤免疫。 PY在啮齿动物中具有肿瘤原性,用灭活的PY转化细胞能诱导肿瘤免疫,故认为这些转化细胞含TSA。被转化细胞早期含三种表达蛋白:大T(LT)、中T(MT)和小T(ST),但它们作为TSA的作用还不知道。因此,作者把这三种T蛋白基因分离出来,分别在 Tumor-specific antigens (TSAs) or their anti-idiotypic antibodies have been used to control the growth of tumors in animal models, but a potent immune response requires the simultaneous presentation of antigens and host tissue compatibility determinants. The authors vaccinated animal models with vaccinia recombinant strains expressing polyomavirus (PY) protein to see if a live vaccinia virus expressing TSA in a host cell can better stimulate tumor immunity. PY is tumorigenic in rodents and transformation of cells with inactivated PY induces tumor immunity, so these transformed cells are thought to contain TSA. Transformed cells contained three early expressed proteins: large T (LT), medium T (MT) and small T (ST), but their role as TSA was not known. Therefore, the authors isolated these three T-protein genes, respectively