论文部分内容阅读
本文作者用~(14)C-亮氨酸观察了 AFT-B_1、AFT-G_1和 AFT-M_1对大鼠肝脏中蛋白质合成的影响,特别着重讨论对线粒体蛋白质合成的作用。体重80—100g 雄性 Wistar 大鼠分别腹腔注射0.2ml 的 AFT-B_1、AFT-G_1和 AFT-M_1二甲亚砜溶液,剂量分别为6、12、6mg/kg,每组6只。24小时后再腹腔注射1-~(14)C 亮氨酸(10μCi/100g 体重),3小时后处死。在体外试验中,大鼠在给与毒素后24小时处死。测线粒体与上清液(胞浆)中的肝蛋白质合成。结果表明,注射 AFT-B_148小时后,肝
The authors used ~ (14) C-leucine to observe the effects of AFT-B_1, AFT-G_1 and AFT-M_1 on protein synthesis in rat livers, with particular emphasis on the effects on mitochondrial protein synthesis. Body weight 80-100g male Wistar rats were intraperitoneal injection of 0.2ml of AFT-B_1, AFT-G_1 and AFT-M_1 dimethyl sulfoxide solution, the dose was 6,12,6 mg / kg, 6 in each group. Twenty-four hours later, intraperitoneal injection of 1- (14) C leucine (10 μCi / 100 g body weight) was given after 3 hours and sacrificed. In in vitro experiments, rats were sacrificed 24 hours after the toxin was administered. Measurement of hepatic protein synthesis in mitochondria and supernatant (cytoplasm). The results showed that liver was injected after AFT-B-148 hours