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目的:探讨花生枝叶对动脉粥样硬化(AS)家兔的干预效果。方法:28只新西兰白兔随机分成两组,其中23只通过注射DL-蛋氨酸造模,5只作正常对照组。4周后剔除造模不成功者后,将造模成功家兔随机分成3组:模型组、辛伐他汀组、花生枝叶组。第5周时,花生枝叶组给予花生枝叶水煎剂灌胃(3.96 g/kg),辛伐他汀组给予辛伐他汀灌胃(5 mg/kg),正常对照组和模型组给予蒸馏水灌胃3 mL,均每日两次。分别在第0、4、8、12周末时检测血清同型半胱氨酸(Hcy)水平和B超检测血管内中膜厚度阻力指数等,12周末处死家兔,HE法检测病理学改变。结果:与正常对照组比较,模型组各时间点Hcy明显升高(P<0.05),内中膜厚度明显增厚(P<0.05),HE染色显示内膜明显增厚,花生枝叶组和辛伐他汀组在各时间点能够不同程度地降低Hcy,减小内中膜厚度(P<0.05),HE染色显示内膜增厚不明显。结论:花生枝叶能降低Hcy,抑制斑块的增长,具有治疗AS的作用。
Objective: To investigate the intervention effects of peanut branches and leaves on atherosclerosis (AS) rabbits. Methods: Twenty-eight New Zealand white rabbits were randomly divided into two groups, of which 23 were injected with DL-methionine and 5 were normal controls. After 4 weeks, the successful models were excluded and the rabbits were randomly divided into 3 groups: model group, simvastatin group and peanut leaf group. At the fifth week, the peanut branches and leaves were given gavage (3.96 g / kg), the simvastatin group was given gavage (5 mg / kg), the normal control group and the model group were given distilled water 3 mL, both twice daily. Serum homocysteine (Hcy) levels and BMI were measured at the 0, 4, 8 and 12weeks respectively. The rabbits were sacrificed at the end of 12th week, and pathological changes were detected by HE method. Results: Compared with the normal control group, the Hcy in the model group was significantly increased at each time point (P <0.05), the thickness of the intima was significantly thicker (P <0.05), and the intima was thicker than the normal control group In the statin group, Hcy decreased at different time points, the thickness of intima decreased (P <0.05), and HE staining showed no significant intimal thickening. Conclusion: The peanut branches and leaves can reduce Hcy, inhibit the growth of plaque, and have the effect of treating AS.