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目的:利用等离子体电解氧化技术,在纯钛表面制备不同锌含量的钙磷活性涂层,比较纯钛表面不同锌含量活性涂层的理化性能差异及其对牙龈卟啉单胞菌(P.gingivalis,Pg)及伴放线放线杆菌(A.actinomycetemcomitans,Aa)抗菌性能的影响。方法:在等离子体电解氧化的电解液中,每组各加入0.08 mol/L钙和0.06 mol/L磷,再分别向各组加入0.01、0.03、0.05 mol/L锌处理纯钛片,制成低、中、高3种不同锌含量的钙磷活性涂层,以不加锌组作为对照组。利用电子万能测试机检测涂层与钛基底的结合力,扫描电镜(SEM)观察钛片表面形貌,X线衍射(XRD)分析材料表面晶相结构,X射线光电子能谱仪(XPS)分析材料表面化学组成及元素成分。材料表面培养Pg和Aa后,应用扫描电镜和贴膜法评价锌含量与抗菌性能的关系。采用SPSS13.0软件包对数据进行统计学分析。结果:随着锌含量的提高,涂层的孔径及粗糙度随之增加;低锌组结晶优于高锌组;低锌组HA和Zn3P2含量较多,高锌组ZnO含量多。扫描电镜观察,随着锌含量的提高,材料表面Pg和Aa细菌数量逐渐减少,高锌组部分细菌出现裂解、坏死。通过贴膜法观察,随着锌含量的提高,材料表面Pg和Aa细菌数量逐渐减少,且4组间差异显著。结论:锌含量的变化会改变涂层的理化性能。高含量锌的钙磷涂层,抗菌性能最佳。
OBJECTIVE: To compare the physico-chemical properties of active zinc coatings with different zinc contents on pure titanium by plasma electrolytic oxidation technique, and to test the effect of Porphyromonas gingivalis gingivalis, Pg) and A.actinomycetemcomitans (Aa). Methods: In the electrolyte of plasma electrolytic oxidation, 0.08 mol / L calcium and 0.06 mol / L phosphorus were added to each group, and then 0.01, 0.03 and 0.05 mol / Low, medium and high three kinds of different levels of calcium and phosphorus coating, with no zinc group as a control group. The bonding force between the coating and the titanium substrate was detected by electronic universal testing machine. The surface morphology of titanium was observed by scanning electron microscope (SEM) and the crystal structure of the surface was analyzed by X-ray diffraction (XRD). The XPS was analyzed by X-ray photoelectron spectroscopy Material surface chemical composition and elemental composition. After the surface of Pg and Aa were cultured on the surface of material, the relationship between zinc content and antibacterial property was evaluated by scanning electron microscopy and film sticking method. SPSS13.0 software package for statistical analysis of the data. Results: With the increase of zinc content, the pore size and roughness of the coatings increased. The crystallization of low zinc group was better than that of high zinc group. The content of HA and Zn3P2 in low zinc group was higher than that in high zinc group. Scanning electron microscopy, with the increase of zinc content, the number of bacteria Pg and Aa on the surface of the material gradually decreased, and some bacteria in the high-zinc group were lysed and necrotic. By the film method observation, with the increase of zinc content, the number of Pg and Aa bacteria on the surface of the material gradually decreased, and the difference between the four groups was significant. Conclusion: Changes in zinc content will change the physical and chemical properties of the coating. High zinc content of calcium phosphate coating, the best antibacterial properties.