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鼠疫耶尔森氏菌的F1抗原,是该菌具有高度特异性的抗原。长期以来,鼠疫细菌的鉴别以及鼠疫的诊断一直依靠检出该种抗原和针对该抗原的抗体。近年来,与F1抗原合成与调节有关的基因已被克隆和测序。 随着PCR与探针技术等分子生物学技术的推广,这些技术也被应用于鼠疫的监测与研究。最初,Mc Donaugh等在鼠疫菌pla基因的基础上,发展了一种特异性的探针。其后,国内外均以此基因为基础,发展了利用PCR诊断鼠疫的技术手段。 笔者根据已发表的F1抗原基因序列,用PCR技术扩增出一个249bp的片段。将此片段标记成为探针,并对这一方法的特异性进行了研究。进一步使用这种探针的结果表明,它总带有低度的非特异性。为此,利用复式PCR技术,对这一探针作了改进。
The F1 antigen of Yersinia pestis is a highly specific antigen of this bacterium. For a long time, the identification of plague bacteria and the diagnosis of plague have always been based on the detection of the antigen and the antibody against the antigen. In recent years, the genes involved in the synthesis and regulation of F1 antigen have been cloned and sequenced. With the promotion of molecular biology techniques such as PCR and probe technology, these techniques have also been applied to the surveillance and research of plague. Originally, McDonaldaugh et al. Developed a specific probe based on the plague plague gene. Subsequently, both at home and abroad are based on this gene, the development of the use of PCR diagnostic plague techniques. According to the published F1 antigen gene sequence, we amplified a 249 bp fragment by PCR. This fragment was labeled as a probe and the specificity of this method was investigated. The results of further use of this probe indicate that it always has a low degree of non-specificity. To this end, the use of duplex PCR technology, this probe has been improved.