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十字花科黑腐病菌(Xanthomonas campestris pv.campestris,Xcc)是研究植物病原细菌和寄主互作的模式细菌,鉴定其致病相关基因对于控制作物病害有重要的意义。XC2038在Xcc 8004中注释为功能未知的假定蛋白基因。本研究利用实验室前期构建的XC2038基因的Tn5gus A5插入突变体164H09,并构建了该突变体的互补菌株C164H09,随后对各菌株的致病性及相关表型进行了检测。结果表明,164H09影响Xcc 8004胞外多糖、泳动性及生物被膜的形成,影响在寄主满身红萝卜上的致病性,而突变体的互补菌株能将以上表型恢复至野生型水平。综上可知,假定蛋白基因XC2038与十字花科黑腐病菌致病相关。
Xanthomonas campestris pv. Campestris (Xcc) is a model organism for studying the interaction between plant pathogenic bacteria and host. Identification of the pathogenicity-related genes is of great significance for the control of plant diseases. XC2038 is annotated in Xcc 8004 as a hypothetical protein of unknown function. In this study, Tn5gus A5 of XC2038 gene pre-lab was inserted into the mutant 164H09, and the complementary strain C164H09 of the mutant was constructed. Then the pathogenicity and related phenotypes of each strain were tested. The results showed that 164H09 affected the extracellular polysaccharides, motility and the formation of biofilm in Xcc 8004, which affected the pathogenicity of the host carrot. However, the complementary strains of mutant could restore the above phenotype to the wild type. In conclusion, we hypothesize that the putative protein gene XC2038 is associated with the pathogenesis of the cruciferous black rot pathogen.