论文部分内容阅读
苹果棉蚜是世界性检疫害虫,在云南省苹果主要产区造成严重的危害.从100条ISSR(Inter-si mple Sequence Repeat)引物中共筛选出4条多态性和重复性好的引物,研究了云南省昆明市、丽江市、昭通市3个苹果主产区共57个采样点的苹果棉蚜种群间及种群内的遗传多样性.4条引物在苹果棉蚜DNA中共扩增出49个位点,多态位点百分率为75.51%.由Shannon信息指数和Nei s指数估算的种群间遗传分化系数为42.48%和17.53%,种群内遗传分化系数分别为57.52%和83.17%,表明苹果棉蚜大部分遗传变异主要存在于种群内部.各种群的遗传距离聚类分析表明,昆明种群与昭通种群的亲缘关系较丽江种群为近.研究结果为我国苹果棉蚜的遗传防治研究提供了分子依据,为进一步研究苹果棉蚜的分子进化提供了背景资料.
The apple aphid is a worldwide quarantine pest, which caused serious harm to the main apple producing areas in Yunnan Province.Four polymorphic and reproducible primers were screened from 100 ISSR primers A total of 57 sampling points in Kunming, Lijiang City, Zhaotong City, Zhaotong City, a total of 57 samples of apple aphids within the cotton aphids population and within the genetic diversity of four primers in the cotton aphid DNA amplification of 49 The percentage of polymorphic loci was 75.51% .The genetic differentiation coefficients estimated by Shannon’s information index and Nei’s index were 42.48% and 17.53%, respectively, and the genetic differentiation coefficients were 57.52% and 83.17% Most of the genetic variation of aphids mainly existed in the population.The genetic distance clustering analysis of each population showed that the genetic relationship between Kunming population and Zhaotong population was closer than that of Lijiang population.The results provided a molecular study on the genetic control of apple aphid in China Based on which provide the background information for further research on molecular evolution of apple aphid.