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目的:研究五参二连颗粒对病毒性心肌炎(VMC)小鼠心肌保护作用及免疫调节机制的研究。方法:用柯萨奇B3型病毒(CoxB3)建立BALB/c小鼠VMC模型。将60只BALB/c小鼠随机分为正常组、模型组、阳性药物利巴韦林组(1 mg·kg-1)及五参二连低、中、高剂量组(4,12,20 mg·kg-1)。造模后第0,1,2,3,4,5天,用药组分别给与相应药物灌胃给药并观察动物一般情况。连续给药5 d,在末次给药2 h后终止实验,称重、并根据检测要求做标本采集。对所取标本进行心肌酶指标检测、脏器指数检测及自然杀伤细胞(NK)杀伤实验。结果:模型组小鼠第2天后开始精神萎靡,活动减少,毛粗糙,体温下降。第5天后处死,心脏晦暗,纹理不清,心脏表面出现白色点状条索状病变。病理学HE染色,心肌组织有大量的炎性细胞浸润,可广泛弥漫,也可呈局灶性,并可有坏死灶。同时模型组的肌酸激酶同工酶、肌钙蛋白、肌红蛋白与正常对照组比较P<0.05,表明造模成功。利巴韦林组,五参二连颗粒高、中剂量组的肌酸激酶同工酶、肌钙蛋白均有下降,与模型对照组比较P<0.05。利巴韦林组及五参二连颗粒高、中剂量组脾指数和胸腺指数有升高,与模型对照组比较P<0.05。利巴韦林及五参二连颗粒高、中剂量组NK杀伤作用与正常对照组和模型对照组比较P<0.05。结论:五参二连颗粒可以减轻CoxB3造成的心肌损伤,并激发机体的免疫调节机制,对心肌细胞有一定保护作用。
Objective: To study the myocardial protective effect of Wushen Erlian Keli on myocarditis in mice with viral myocarditis (VMC) and the mechanism of immune regulation. Methods: VMC model of BALB / c mice was established by Coxsackievirus B3 (CoxB3). 60 BALB / c mice were randomly divided into normal group, model group, positive drug ribavirin group (1 mg · kg-1) and low-dose, middle-dose and high- mg · kg-1). After 0, 1, 2, 3, 4 and 5 days after modeling, the drug groups were administered with the corresponding drugs gavage and observed the general situation of the animals. Continuous administration of 5 d, 2 h after the last dose termination of the experiment, weighed, and according to test requirements to do specimen collection. The samples taken for detection of myocardial enzyme index, organ index test and natural killer (NK) killing experiments. Results: Mice in model group began to be apathetic, decreased in activity, rough in hair and decreased in body temperature after 2 days. After the 5th day of sacrifice, the heart is dull, the texture is unclear, and white spot-like cord-like lesions appear on the surface of the heart. Pathological HE staining, myocardial tissue with a large number of inflammatory cell infiltration, can be widely diffuse, but also showed focal, and may have necrotic lesions. At the same time model group, creatine kinase isoenzyme, troponin, myoglobin compared with the normal control group P <0.05, indicating that modeling success. In ribavirin group, the creatine kinase isoenzyme and troponin of Wenshen Erlian Granules in high and medium dose groups all decreased, P <0.05 compared with model control group. The spleen index and thymus index of ribavirin group and Wuxian Erlian Granules in high dose and medium dose group were higher than those in model control group (P <0.05). The killing effect of ribavirin and Wuxian Erlian Granules in medium and high dose NK group was significantly lower than that in normal control group and model control group (P <0.05). Conclusion: Wuxian Erlian Granule can relieve the myocardial injury caused by CoxB3 and stimulate the immune regulation mechanism of the body, which has a certain protective effect on cardiomyocytes.