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对２５例大肠癌根治术患者的新鲜标本进行细胞分离和原代培养，采用噻唑氮蓝（ＭＴＴ）比色分析法和氚胸腺嘧啶核苷（３ＨＴｄＲ）掺入法，在体外观察５肽胃泌素（ＰＧ）对原代培养大肠癌活细胞数（吸光度，Α值）和ＤＮＡ合成（脉冲数，ＣＰＭ值）的影响。结果：ＰＧ组高、中及低分化腺癌活细胞数和ＤＮＡ合成均明显高于对照组（Ｐ＜０．０１，Ｐ＜０．０５）。出现明显促细胞生长所需ＰＧ浓度高分化腺癌组为０．３９０６μｇ／ｍｌ，而中、低分化腺癌组均为６．２５００μｇ／ｍｌ。本研究结果提示：胃泌素可促进人大肠癌细胞的增殖，尤以高分化腺癌对胃泌素更敏感。这为大肠癌患者特别是高分化腺癌应用胃泌素受体拮抗剂进行内分泌治疗提供了实验依据。
The cell separation and primary culture of fresh specimens from 25 patients with colorectal cancer undergoing radical resection were performed in vitro using thiazolidine blue (MTT) colorimetric assay and thymidine thymidine (3H-TdR) incorporation method. The effect of peptide gastrin (PG) on the number of living cells (absorbance, enthalpy) and DNA synthesis (number of pulses, CPM) of primary cultured colorectal cancer. Results: The number of viable cells and DNA synthesis of high, moderate and poorly differentiated adenocarcinoma in PG group were significantly higher than those in control group (P<0.01, P<0.05). The PG concentration in the well-differentiated adenocarcinoma group that significantly promoted cell growth was 0.3906 μg/ml, while the moderately-differentiated adenocarcinoma group was 6.2500 μg/ml. The results of this study suggest that gastrin can promote the proliferation of human colorectal cancer cells, especially in highly differentiated adenocarcinomas that are more sensitive to gastrin. This provides an experimental basis for the application of gastrin receptor antagonists for endocrine therapy in colorectal cancer patients, especially well-differentiated adenocarcinomas.