论文部分内容阅读
The paper describes a controllable layer-by-layer(LBL)self-assembly modification technique of multi-walled carbon nanotubes (MWNTs)and poly(diallyldimethylammonium chloride)(PDDA)towards glassy carbon electrode(GCE),Acetylcholinesterase (AChE)was immobilized directly to the modified GCE by LBL self-assembly method,the activity value of AChE was detected by using i-t technique based on the modified Ellman method.Then the composition of carbaryl were detected by the enzyme electrode with 0.01 U activity value and the detection limit of carbaryl is 10~(-12) g L~(-1) so the enzyme biosensor showed good properties for pesticides residue detection.
The paper describes a controllable layer-by-layer (LBL) self-assembly modification technique of multi-walled carbon nanotubes (MWNTs) and poly (diallyldimethylammonium chloride) (PDDA) towards glassy carbon electrode (GCE), Acetylcholinesterase (AChE) was immobilized directly to the modified GCE by LBL self-assembly method, the activity value of AChE was detected by using it technique based on the modified Ellman method. Chen the composition of carbaryl were detected by the enzyme electrode with 0.01 U activity value and the detection limit of carbaryl is 10 ~ (-12) g L ~ (-1) so the enzyme biosensor showed good properties for pesticides residue detection.