论文部分内容阅读
目的通过对凝固酶阴性葡萄球菌(CNS)菌株进行分型以示CNS菌种间及菌种内的异质性和亲缘性,协助新生儿败血症的诊断。方法将本院新生儿病房2001年11月至2003年3月血培养为CNS的患儿列入本研究。对入选的CNS菌株用脉冲电场凝胶电泳方法分离菌株。结果共有80例患儿入选,其中9例患儿分离出≥2株CNS菌株。血培养为CNS的临床分离菌株共有90株。在临床诊断的27例(33.8%)败血症中,表皮葡萄球菌18例(66.7%),溶血葡萄球菌7例(25.9%),人葡萄球菌2例(7.4%)。脉冲电场凝胶电泳对90株入选菌株共分为50型,其中表皮葡萄球菌37型,溶血葡萄球菌10型,人葡萄球菌2型,华纳葡萄球菌1型。在临床分离的90株CNS菌株中,共有6组图形完全一致,属于同一菌株。其中两组(4株CNS菌株)分别来自2例患儿不同时期的血培养结果,故脉冲电场凝胶电泳可以协助临床明确诊断败血症。结论脉冲电场凝胶电泳是一种敏感性高的CNS菌株分型方法,可协助临床诊断败血症。
Objective To assist in the diagnosis of neonatal sepsis by genotyping coagulase negative staphylococci (CNS) strains to show heterogeneity and relatives within and between CNS strains. Methods The neonatal ward in our hospital from November 2001 to March 2003 were enrolled in this study. Strains were isolated from the selected CNS strains by pulsed-field gel electrophoresis. Results A total of 80 children were enrolled. Among them, ≥2 strains of CNS were isolated from 9 children. There are 90 clinical isolates of blood culture CNS. Of 27 (33.8%) sepsis clinically diagnosed, 18 were Staphylococcus epidermidis (66.7%), 7 were Staphylococcus hemolyticus (25.9%) and 2 were Staphylococcus aureus (7.4%). Ninety isolates were divided into 50 types by pulse electric field gel electrophoresis, including Staphylococcus epidermidis 37, Staphylococcus haemolyticus 10, Staphylococcus human 2, and Staphylococcus aureus 1. Among the 90 clinically isolated strains of CNS, a total of 6 sets of patterns were identical and belonged to the same strain. The two groups (4 strains of CNS) were from two different stages of blood culture results, so that pulse electric field gel electrophoresis can help clinical diagnosis of sepsis. Conclusions Pulsed-field gel electrophoresis is a sensitive method for typing of CNS strains, which can be used to diagnose sepsis clinically.