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目的探讨与肿瘤转移相关的蛋白在乳腺浸润性导管癌中的表达及其意义。方法应用组织芯片技术及SP免疫组化方法检测247例乳腺浸润性导管癌中α-B晶体蛋白,CD44v6,MMP-2,TIMP-2的表达,并分析它们与乳腺癌临床病理特征的关系。结果(1)在浸润性导管癌中,α-B晶体蛋白,CD44v6,MMP-2,TIMP-2的阳性表达率分别为70.0%,61.5%,57.5%,57.1%;与正常组织比较差异有统计学意义(P<0.001);(2)单因素分析显示α-B晶体蛋白,CD44v6,MMP-2的表达与淋巴结转移呈正相关(r=0.317,0.623,0.705),而TIMP-2的表达与之呈负相关(r=-0.532);(3)多因素分析显示淋巴结转移与α-B晶体蛋白,CD44v6,MMP-2,TIMP-2的表达相关,该4项指标联合对淋巴结转移的预测率达89.5%。结论α-B晶体蛋白,CD44v6,MMP-2,TIMP-2的表达情况与乳腺浸润性导管癌的转移密切相关,通过对原发瘤多种转移相关蛋白的检测可以预测乳腺癌的转移能力。
Objective To investigate the expression of tumor associated protein in breast invasive ductal carcinoma and its significance. Methods The expression of α-B crystallin, CD44v6, MMP-2 and TIMP-2 in 247 cases of invasive ductal carcinomas of the breast was detected by tissue microarray and SP immunohistochemistry. The relationship between them and the clinicopathological features of breast cancer was analyzed. Results (1) The positive rates of α-B crystal protein, CD44v6, MMP-2 and TIMP-2 in invasive ductal carcinoma were 70.0%, 61.5%, 57.5% and 57.1% respectively. (2) The expression of α-B crystal protein, CD44v6 and MMP-2 was positively correlated with lymph node metastasis (r = 0.317,0.623,0.705), while the expression of TIMP-2 (R = -0.532). (3) Multivariate analysis showed that lymph node metastasis was related to the expression of α-B crystal protein, CD44v6, MMP-2 and TIMP-2. The forecast rate of 89.5%. Conclusion The expression of α-B crystal protein, CD44v6, MMP-2 and TIMP-2 are closely related to the metastasis of invasive ductal carcinoma. The metastatic capacity of breast cancer can be predicted by the detection of multiple metastasis-associated proteins in primary tumor.