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目的 观察调肝导浊中药对大鼠实验性高脂血症及主动脉平滑肌细胞增殖的影响。方法 采用高胆固醇饮食饲养 SD大鼠 2个月 ,造成高脂血症模型 ,观察中药对大鼠血脂质的影响。此外体外培养大鼠主动脉平滑肌细胞 (SMC) ,以加高脂血清和加中药等不同培养基分组培养 ,取细胞观察中药对 SMC增殖的调控作用。结果 (1 )中药能明显降低血胆固醇、甘油三酯、LDL含量 ,升高 HDL及其亚型含量。(2 )中药可明显抑制高脂血清培养下 SMC的增殖 ,其 PCNA阳性率低于造模组。结论 调肝导浊中药可有效地调整脂蛋白代谢 ,抑制 SMC增殖 ,从而防治高脂血症 ,阻止AS发生发展。
Objective To observe the effect of Tiaogan Zhiliu Chinese herbs on experimental hyperlipidemia and aortic smooth muscle cell proliferation. Methods SD rats were fed with high cholesterol diet for 2 months, resulting in hyperlipidemia model and observing the effect of traditional Chinese medicine on blood lipid of rats. In addition, rat aortic smooth muscle cells (SMCs) were cultured in vitro, and the cells were cultured in different medium, such as increasing serum lipids and adding traditional Chinese medicine. The cells were observed for the regulation of SMC proliferation. Results (1) Chinese medicine can significantly reduce blood cholesterol, triglycerides, LDL content, increased HDL and its subtypes. (2) Chinese medicine can significantly inhibit the proliferation of SMC in hyperlipidemic serum, the positive rate of PCNA is lower than the model group. Conclusion Tiaogan Zhiliu Chinese medicine can effectively regulate lipoprotein metabolism, inhibit the proliferation of SMC, thus preventing and treating hyperlipidemia and prevent the development of AS.