论文部分内容阅读
目的 :以芍药苷和芍药内酯苷为指标成分,建立白芍总苷血浆蛋白结合率的测定方法,比较其在CCl4肝损伤大鼠及正常大鼠血浆中蛋白结合率的异同。方法 :采用大鼠血浆平衡透析法,HPLC法测定透析袋两侧溶液中芍药苷和芍药内酯苷的质量浓度,分别计算其在CCl4肝损伤大鼠血浆中和正常大鼠血浆中的蛋白结合率。结果 :白芍总苷血浆浓度为50,100和200μg/ml时,芍药苷与肝损伤大鼠血浆的蛋白结合率分别为(15.5±0.4)%、(16.5±1.4)%、(27.9±1.6)%,与正常大鼠血浆的蛋白结合率分别为(12.7±1.6)%、(13.5±0.8)%、(15.6±0.6)%;芍药内酯苷与肝损伤大鼠血浆的蛋白结合率分别为(12.4±1.5)%、(13.2±1.6)%、(20.2±2.1)%,与正常大鼠血浆的蛋白结合率分别为(8.1±0.9)%、(9.7±2.3)%、(14.7±1.4)%。结论 :白芍总苷中主要成分芍药苷和芍药内酯苷与CCl4肝损伤大鼠及正常大鼠血浆的蛋白结合均属低度结合,且与血药浓度有一定的依赖性,CCl4肝损伤后大鼠血浆蛋白结合率较正常大鼠提高,提示白芍总苷临床应用时可能受患者肝功能状态的影响。
OBJECTIVE: To establish a method for the determination of plasma protein binding of paeoniflorin by using paeoniflorin and paeoniflorin as index components, and to compare the protein binding rates of paeoniflorin and paeoniflorin in the plasma of CCl4-injured rats and normal rats. Methods: The concentration of paeoniflorin and paeoniflorin in solution on both sides of the dialysis bag were measured by rat plasma dialysis method and HPLC respectively. The protein concentrations of paeoniflorin and paeoniflorin were calculated respectively in plasma of rats with CCl4 hepatic injury and normal rats rate. Results: The plasma protein binding rates of paeoniflorin and hepatic injury rats were (15.5 ± 0.4)%, (16.5 ± 1.4)% and (27.9 ± 1.6)%, respectively, at plasma concentrations of 50, 100 and 200 μg / (12.7 ± 1.6)%, (13.5 ± 0.8)%, (15.6 ± 0.6)%, respectively. The protein binding rate of plasma between rats with and without paeoniflorin was ( (8.1 ± 0.9)%, (9.7 ± 2.3)%, (14.7 ± 1.4)% and (12.4 ± 1.5)%, (13.2 ± 1.6)% and %. Conclusion: The main constituents of paeoniflorin paeoniflorin and albiflorin and CCl4 hepatic injury in rats and normal plasma protein binding are low binding, and with a certain degree of plasma concentration dependence, CCl4 liver injury The plasma protein binding rate of rats was higher than that of normal rats, suggesting that the clinical application of total glucosides of paeony may be affected by the state of liver function.