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目的:观察赤雹果总有机酸(FTDBP)对大鼠离体子宫平滑肌活力的影响。方法:选用8周龄Wistar雌性未孕大鼠,以子宫平滑肌的收缩频率、收缩幅度指标,计算子宫平滑肌活力及FTDBP的半数抑制浓度(IC50)。以盐酸普萘洛尔、盐酸苯海拉明、盐酸雷尼替丁、硫酸阿托品为阻断剂,观察FTDBP对子宫平滑肌作用与β,H1,H2,M受体的关系,探讨FTDBP对子宫平滑肌的作用机制。结果:与模型组比较,FTDBP剂量组(0.032,0.064,0.128 g·L-1)和元胡止痛片组对缩宫素所致大鼠离体子宫平滑肌的收缩频率、收缩幅度和活力均有明显的抑制作用(P<0.05或P<0.01),IC50为0.100 24 g·L-1;与模型组比较,硫酸阿托品组大鼠子宫平滑肌的收缩频率、幅度和活力差异无统计学意义。结论:FTDBP对大鼠子宫平滑肌活力有明显的抑制作用,其作用是通过抑制M受体实现的,与H1,H2,β受体无关。
Objective: To observe the effect of FTDBP on the vitality of isolated uterine smooth muscle in rats. Methods: Uterus of 8 weeks old Wistar female rats were selected. The contractile frequency and amplitude of uterine smooth muscle were measured to determine the activity of uterine smooth muscle and the half maximal inhibitory concentration (FT-IC50) of FTDBP. The effects of FTDBP on the uterine smooth muscle function and β, H1, H2, M receptors were observed with propranolol hydrochloride, diphenhydramine hydrochloride, ranitidine hydrochloride and atropine sulfate. The effects of FTDBP on uterine smooth muscle The mechanism of action. Results: Compared with the model group, FTDBP dose group (0.032,0.064,0.128 g · L-1) and Yuanhu Zhitong tablet group on the uterine contraction of rat uterine smooth muscle contraction frequency, contraction amplitude and vitality (P <0.05 or P <0.01), IC50 was 0.100 24 g · L-1. Compared with the model group, there was no significant difference in the contraction frequency, amplitude and activity of uterine smooth muscle in atropine sulfate group. Conclusion: FTDBP significantly inhibits the activity of uterine smooth muscle in rats, and its effect is mediated by inhibition of M receptor, but not with H1, H2 and β receptors.