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采自幼树、成年树及成年树伐根萌条的嫩梢腋芽,在改良DKW培养基+6-BA1.0mg/L中可伸长生长;在加有6-BA1.0mg/L+IBA 0.01mg/L的继代培养基中,可保持腋芽的生长并形成不定芽。芽增殖率为每月600％左右。芽苗经5.0mg/L IBA处理7天,然后在含活性炭的无激素培养基中培养20天,有54％可生根成苗。5月中旬至6月上旬的幼胚,在改良DKW培养基+6-BA1.0 mg/L中,黑暗培养30天左右可产生体细胞胚,并可连续多代保持胚性分生能力。体胚在无激素的发芽培养基中黑暗培养7天左右可发芽成苗。胚性愈伤组织在悬浮振荡培养中可保持分生能力。
Taken from the young tree, adult tree and adult tree prickly bud young axillary buds, improved DKW medium + 6-BA1.0mg / L can be elongated growth; add 6-BA1.0mg / L + IBA 0.01mg / L subculture medium, can maintain the growth of axillary buds and the formation of adventitious buds. Bud proliferation rate of about 600% per month. Sprouts were treated with 5.0 mg / L IBA for 7 days and then cultured for 20 days in hormone-free medium containing activated charcoal, 54% of which could be rooted. The immature embryos from mid-May to early June can produce somatic embryos in a modified DKW medium + 6-BA1.0 mg / L for about 30 days in darkness and can retain embryogenic meristematic ability for many generations. Somatic embryos in hormone-free germination medium in the dark about 7 days can germinate into seedlings. Embryogenic callus maintains viability in suspension culture.