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目的观察别旁茶(BPC)总苷对溃疡性结肠炎(UC)小鼠模型干预的药效作用,考察不同分组小鼠的尿液代谢表达谱的差异。方法 balb/c小鼠随机分为BPC组、三硝基苯磺酸(TNBS)干预组、正常对照组和阳性药组,药物干预后测定药效指标,采用超高效液相色谱-四级杆飞行时间质谱(UPLC/Q-TOf MS)分析动物尿样,数据处理采用主成分分析(PCA)和偏最小二乘判别法(PLSDA)。结果与模型组比较,BPC组小鼠结肠组织损伤明显减轻,SOD活性升高(P<0.01),MPO活性和MDA水平明显降低(P<0.01)。代谢组学模式识别分析各组区别明显,PLSDA方法区分尤为明显。结论代谢组学初步研究显示,别旁茶总苷可明显改善溃疡性结肠炎小鼠模型的病变,PLSDA代谢组学数据处理方法可靠。
Objective To observe the pharmacodynamic effects of glycosides of BPC on mouse models of ulcerative colitis (UC) and to investigate the difference of urinary metabolites in different groups of mice. Methods Balb / c mice were randomly divided into BPC group, TNBS intervention group, normal control group and positive drug group. The pharmacodynamic parameters were determined after drug intervention. The ultra performance liquid chromatography-quadrupole Animal urine samples were analyzed by time-of-flight mass spectrometry (UPLC / Q-TOF MS) using principal component analysis (PCA) and partial least squares discriminant (PLSDA). Results Compared with model group, the damage of colonic tissue in BPC group was significantly reduced, the activity of SOD was increased (P <0.01), the activity of MPO and the level of MDA were decreased significantly (P <0.01). Metabolomics pattern recognition analysis of each group was significantly different, PLSDA method is particularly evident. Conclusion Preliminary metabonomics studies have shown that albinoside can significantly improve the pathological changes in mouse models of ulcerative colitis. The data of PLSDA metabolomics are reliable.