目的 (1)观察选择性地抑制核因子-κB(NF-κB)活性对 K562细胞凋亡的影响;(2)观察蛋白酶体抑制剂MG132对 NF-κB活性及凋亡抑制蛋白 survivin 表达的影响;(3)探讨 NF-κB活性及凋亡抑制蛋白 survivin 表达的相互关系;(4)探讨 NF-κB信号转导通路的调节对白血病细胞凋亡的可能机制。方法细胞系为人髓系白血病细胞 Objective (1) To observe the effect of selectively inhibiting the activity of nuclear factor-κB (NF-κB) on the apoptosis of K562 cells. (2) To observe the effect of proteasome inhibitor MG132 on the activity of NF-κB and the expression of survivin ; (3) to explore the relationship between the activity of NF-κB and the expression of survivin protein; (4) to explore the possible mechanism of NF-κB signal transduction pathway in the apoptosis of leukemic cells. Methods The cell line is human myeloid leukemia cells