本文作者在肯尼亚5种不同类型的地区用利什曼抗原进行了Montenegro皮内反应试验。采用的抗原为热带利什曼原虫的培养物。培养物经以3,000转/分离心10分钟后,去其上清液,将鞭毛体混悬于无菌生理盐水中,再以盐水混悬离心,如此洗涤3次。经离心浓集的鞭毛体悬液,以0.5%的石炭酸无菌生理盐水稀释,并通过微孔滤器过滤,最后抗原的稀释液为每毫升含有鞭毛体1×10~6 The authors performed a Montenegro intradermal reaction with Leishman’s antigen in five different types of kenya. The antigen used is a culture of Leishmania tropica. After the culture was centrifuged at 3,000 rpm for 10 minutes, the supernatant was removed, the flagellar body was suspended in sterile physiological saline, and then centrifuged in saline for three times. After centrifugation concentrated flagellar suspension, 0.5% sterile saline solution of carbolic acid and filtered through a microporous filter, the final dilution of the antigen per ml flagellated body 1 × 10 ~ 6