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本文用Ｆｏｓ蛋白免疫组织化学方法研究了大鼠脊髓内接受盆腔脏器伤害性刺激神经元的分布状况。在对照组，仅偶见Ｆｏｓ阳性细胞出现于Ｌ６、Ｓ１节段脊髓后角（＜２个／片），且染色浅淡。将３％福尔马林经插管分别注入膀胱、阴道和直肠造成伤害性刺激时，Ｆｏｓ阳性细胞数明显增多（１００～２６０个／片，Ｓ１），主要出现于Ｌ６、Ｓ１节段后角Ⅰ层内侧部、后角内侧缘、后连合核和中间带外侧核。少量散在于后角Ⅰ层外侧部、后角外侧缘、外侧脊核和前角背内侧部。为了确定脊髓内Ｆｏｓ阳性神经元是否向上位脑结构投射，将荧光金（ＦＧ）注入一侧臂旁外侧核后，给予膀胱伤害性刺激，结合Ｆｏｓ蛋白免疫组化技术，在后连合核和中间带外侧核发现有ＦＧ／Ｆｏｓ双标细胞。在中间带外侧核，ＦＧ标记细胞与Ｆｏｓ阳性细胞均主要位于核的背侧部，且ＦＧ标记细胞多数同时为Ｆｏｓ阳性，ＦＧ／Ｆｏｓ双标细胞占ＦＧ标记细胞总数的７１．２％（３７／５２），占Ｆｏｓ阳性细胞总数的１４．８％（３７／２５０），提示脊髓内接受盆腔脏器伤害性神经元部分投射至臂旁外侧核。为进一步确定Ｆｏｓ阳性细胞在中间带外侧核定位分布特征，采用ＮＡＤＰＨ脱氢酶反应（显示副交感节前神经元）与Ｆｏｓ蛋白免疫组化相结合?
Fos protein immunohistochemistry was used to study the distribution of nociceptive neurons in the spinal cord of rats receiving pelvic organ. In the control group, occasional Fos positive cells appeared in the posterior horn (<2 pieces / piece) of L6 and S1 segments, and the staining was light. When 3% formalin was injected intrathecally into the bladder, vagina and rectum to cause noxious stimulation, the number of Fos positive cells increased obviously (100-260 pcs per piece, S1), mainly in the posterior horn of L6 and S1 segments The inner part of the layer Ⅰ, the medial edge of the posterior horn, the posterior commissural nucleus and the medial lateral ventricle. A small amount of scattered in the posterior horn I lateral part, posterior horn outer edge, lateral spinal dorsal horn and anterior horn. In order to determine whether the spinal cord Fos-positive neurons projecting to the superior brain structure, the fluorescence of gold (FG) injected into the lateral arm of the parabrachialis, giving the bladder noxious stimulation, combined with Fos protein immunohistochemistry in the posterior commissure and The middle of the lateral nucleus found FG / Fos double labeled cells. In the middle lateral nucleus, both FG-labeled cells and Fos-positive cells were mainly located in the dorsal part of the nucleus. Most of the FG-labeled cells were positive for Fos, and FG / Fos double-labeled cells accounted for 71.2% of the total number of FG- / 52), which accounted for 14.8% (37/250) of the total number of Fos positive cells, suggesting that some of the nociceptive neurons in pelvic organs were projected onto the lateral parabrachial nucleus. In order to further determine the localization of Fos-positive cells in the lateral medullary nucleus, NADPH dehydrogenase reaction (showing parasympathetic preganglionic neurons) was combined with Fos protein immunohistochemistry.