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目的探究piwil2、乳胶素和piR-932在白血病干细胞中的表达情况,为对成人急性髓性白血病的管理打下基础。方法应用流式细胞术挑选CD34+/CD38-白血病细胞。通过PCR分析技术检测piwil2和胶乳素在CD34+/CD38-白血病细胞中的表达。用piRNA微阵列芯片试验检测piR932在CD34+/CD38-白血病细胞中的表达。结果 piwil2蛋白的表达高于对照组,一种叫作piR932的piRNAs在白血病干细胞中表达显著升高,通过与piwil2发生免疫反应而形成免疫复合物;胶乳素的表达由于其启动子区的Cp G岛的甲基化而大大降低。结论 piR932和piwil2的结合是通过促进胶乳素的甲基化在CD34+/CD38-白血病细胞的过程中起到正性调节的作用,而且它们都能成为白血病的潜在靶点。
Objective To investigate the expression of piwil2, lutein and piR-932 in leukemia stem cells and lay the foundation for the management of adult acute myeloid leukemia. Methods CD34 + / CD38- leukemic cells were selected by flow cytometry. The expression of piwil2 and lactalbumin in CD34 + / CD38- leukemia cells was examined by PCR analysis techniques. PiR932 was detected in CD34 + / CD38- leukemic cells by piRNA microarray chip assay. Results The expression of piwil2 protein was higher than that of the control group. A piRNAs named piR932 expressed significantly higher in leukemia stem cells and formed an immune complex by immunoreaction with piwil2. The expression of calpain was regulated by its CpG Island methylation and greatly reduced. Conclusions The binding of piR932 to piwil2 plays a positive regulatory role in CD34 + / CD38- leukemia cells by promoting the methylation of the latex, and both of them can be potential targets for leukemia.