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目的探讨ITGB3基因SNP与阿司匹林抗血小板效应的相关性。方法选择宁波市第一医院及宁波市姜山镇社区服务中心规律服用阿司匹林100mg/d两周以上的心血管疾病患者238例,采集外周静脉血,通过Veri-fyNow-Aspirin方法评估其血小板反应性,结果以ARU表示。同时提取静脉血全基因组DNA,采用Snapshot方法检测ITGB3基因SNPrs11871251和rs2317676;并通过ELISA法检测其尿11-DH-TXB2浓度,分析SNP与阿司匹林抗血小板效应的联系。结果本研究人群中ITGB3基因rs11871251基因型频率分别为AA 22.5%、AG52.4%及GG 25.3%;rs2317676基因型频率分别为GG 4.3%、GA 29.4%及AA 66.2%。rs2317676不同基因型携带者ARU值差异有统计学意义(<0.05),而其尿11-DH-TXB2水平差异无统计学意义(>0.05);SNPrs11871251不同基因型携带者ARU及尿11-DH-TXB2水平差异均无统计学意义(>0.05)。不同SNP基因型携带者高血压、糖尿病及血脂异常患病率差异均无统计学意义(>0.05)。结论 ITGB3基因SNP rs2317676可能与阿司匹林抗血小板效应有关,研究结果有待大样本量人群资料进一步支持。
Objective To investigate the relationship between the ITGB3 gene SNP and anti-platelet effect of aspirin. Methods The first hospital of Ningbo City and Ningbo City, Jiangshan community service center regularly taking aspirin 100mg / d for more than two weeks in 238 patients with cardiovascular disease, peripheral venous blood collected by Veri-fyNow-Aspirin method to assess its platelet reactivity, Results are expressed as ARU. Genomic DNA of venous blood was extracted simultaneously. The ITGB3 gene SNP rs11871251 and rs2317676 were detected by Snapshot method. The urinary 11-DH-TXB2 concentration was detected by ELISA. The relationship between SNP and anti-platelet effect of aspirin was analyzed. Results The frequencies of rs11871251 genotype of ITGB3 gene in this study were AA 22.5%, AG 52.4% and GG 25.3%, respectively. The frequencies of rs2317676 genotype were GG 4.3%, GA 29.4% and AA 66.2%, respectively. There was no significant difference in urinary 11-DH-TXB2 levels between the two groups (rs2317676, ARU) and ARU (P> 0.05) TXB2 levels were no significant difference (> 0.05). There was no significant difference in prevalence of hypertension, diabetes and dyslipidemia among different SNP genotype carriers (> 0.05). Conclusion The SNP rs2317676 of ITGB3 gene may be related to the antiplatelet effect of aspirin, and the results of this study are yet to be further supported by the large sample size of population data.