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目的 探讨肾移植患者术后外周血CD3+细胞CD6 9、CD2 5及CD71的表达及其意义。方法 提取患者的外周血淋巴细胞 ,进行CD3·PE和CD6 9/CD2 5 /CD71·FITC的双色免疫荧光标记 ,流式细胞分析仪测定。结果 术后稳定组和环孢素中毒组CD3+细胞CD6 9、CD2 5及CD71的表达均较术前下降 (P <0 .0 5 ) ,而急性排斥反应组和感染组则显著升高 (P <0 .0 1) ,而且其升高的时间比临床确诊要早 1～ 4d。结论 动态监测CD3+细胞CD6 9、CD2 5及CD71的表达将有助于急性排斥的早期诊断和鉴别诊断 ,对及时治疗和抗排斥疗效的评价具有一定意义。
Objective To investigate the expression and significance of CD6 9, CD25 and CD71 in peripheral blood CD3 + cells after renal transplantation. Methods Peripheral blood lymphocytes of patients were extracted and double-labeled immunofluorescent labeling of CD3 · PE and CD6 9 / CD2 5 / CD71 · FITC was performed. Flow cytometric analysis was performed. Results The expression of CD69, CD25 and CD71 in the CD3 + cells of the stable group and cyclosporine poisoning group were significantly lower than those before operation (P <0.05), but significantly higher in the acute rejection group and the infected group (P <0. 01), and its rise time than the clinical diagnosis of early 1 ~ 4d. Conclusion The dynamic monitoring of the expression of CD6 9, CD2 5 and CD71 in CD3 + cells will be helpful for the early diagnosis and differential diagnosis of acute rejection, which is of great significance for the evaluation of timely treatment and anti-rejection efficacy.