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目的研究分离自海洋沉积物的疣孢菌FIM090395产生的次级代谢产物。方法采用HP-20大孔吸附树脂、正相硅胶、Sephadex LH-20葡聚糖凝胶和反相C18柱层析等方法,对菌株FIM090395次级代谢产物进行分离纯化。通过紫外光谱、质谱、核磁共振等波谱学方法对化合物进行结构鉴定。结果与结论分离得到化合物1和化合物2,结构解析确定它们分别与proximicin A和B同质,活性研究表明化合物1对肿瘤细胞株MDA-MB-231(乳腺癌细胞)、SW620(结肠癌细胞)、SMMC7721(肝癌细胞)和CNE-2(鼻咽癌细胞)具有一定的抑制作用,其IC50值分别为23.49、13.48、34.96和16.28μg/mL;化合物2对肿瘤细胞株K562(白血病细胞)具有一定的抑制作用,其IC50值为25.69μg/mL,对MDA-MB-231和CNE-2的抑制增殖作用较弱。
Objective To study the secondary metabolites produced by Verruconi FIM090395 isolated from marine sediments. Methods The secondary metabolites of strain FIM090395 were isolated and purified by HP-20 macroporous adsorption resin, normal phase silica gel, Sephadex LH-20 Sephadex G-30 and reverse phase C18 column chromatography. The structures of the compounds were identified by UV spectroscopy, mass spectrometry and nuclear magnetic resonance spectroscopy. RESULTS AND CONCLUSIONS Compounds 1 and 2 were isolated and their structures were identified as being homogeneous with proximicin A and B, respectively. Activity studies showed that compound 1 exerted its effects on tumor cell lines MDA-MB-231 (breast cancer cells), SW620 (colon cancer cells) , SMMC7721 (hepatocellular carcinoma cells) and CNE-2 (nasopharyngeal carcinoma cells) with IC50 values of 23.49, 13.48, 34.96 and 16.28μg / mL, respectively. Compound 2 had inhibitory activity on tumor cell line K562 (leukemia cells) The IC50 value was 25.69μg / mL, which showed a weak inhibitory effect on the proliferation of MDA-MB-231 and CNE-2.