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为了明确长枝木霉Trichoderma longibrachiatum对小麦禾谷胞囊线虫Heterodera avenae卵的防治潜力和作用机制,在室内测定了长枝木霉分生孢子悬浮液对小麦禾谷胞囊线虫卵的寄生和毒杀作用,并通过温室盆栽试验测定了长枝木霉分生孢子悬浮液对小麦禾谷胞囊线虫卵的防治效果。室内试验中1.5×107CFU/mL的长枝木霉分生孢子悬浮液处理12 d对小麦禾谷胞囊线虫卵的寄生率和卵孵化的相对抑制率分别为91.33%和88.35%。温室盆栽试验中1.5×107CFU/mL处理对土壤中胞囊、线虫和根系中线虫的相对防治效果分别为89.84%、92.67%和88.30%;接种卵悬浮液和长枝木霉分生孢子悬浮液后,小麦幼苗株高、根系长度、地上部鲜重和根系鲜重的增长率分别为66.72%、159.31%、169.09%和170.19%;叶绿素总量和根系活力显著高于对照,分别为17.52 mg/g和356.25μg·g-1·h-1。研究表明长枝木霉可作为小麦禾谷胞囊线虫的生防制剂。
In order to clarify the control potential and mechanism of trichoderma longibrachiatum against Heterodera avenae eggs in wheat, the parasitism and toxicity of the Trichoderma longum conidia suspension on wheat cereal cyst nematode eggs were determined in laboratory Toxic effects, and through the greenhouse pot experiment measured Tricholoma longum conidia suspension of wheat cereal cyst nematode egg control effect. In laboratory experiments, the parasitism rate and egg hatching inhibition rate of wheat xylostella nematode eggs were 91.33% and 88.35% respectively after being treated with 1.5 × 107CFU / mL of Trichoderma viride conidia suspension for 12 days. The relative control effects of 1.5 × 107CFU / mL treatment on soil cysts, nematodes and nematodes in the soil were 89.84%, 92.67% and 88.30% respectively in potted plants in greenhouse. The inoculation of egg suspension and Trichoderma viride conidia suspension The growth rates of plant height, root length, fresh shoot weight and root fresh weight of wheat were 66.72%, 159.31%, 169.09% and 170.19% respectively. The total chlorophyll and root activity were significantly higher than those of the control, which were 17.52 mg / g and 356.25 μg · g-1 · h-1. Studies have shown that Trichoderma longibrachiatum can be used as a bio-control agent of wheat and cyst nematode.