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目的：探讨新疆民、汉族喉乳头状瘤及喉鳞状上皮细胞癌的发生与发展过程中，ｐ５３ 蛋白及ＰＣＮＡ 表达的关系及临床病理学意义。方法：应用免疫组织化学方法对６３ 例喉乳头状瘤（维吾尔族３３ 例，汉族３０ 例），８３例喉鳞状上皮细胞癌（维吾尔族２７ 例，哈萨克族４例，汉族５２ 例），２０ 例声带息肉（维吾尔族１０ 例，汉族１０ 例）分别进行检测。结果：（１）喉鳞状上皮细胞癌组织中突变型ｐ５３ 蛋白阳性率为６２．７％ （５２／８３），明显高于喉乳头状瘤的２８．５％ （２４／６３）和声带息肉的０．０％ （０／２０）（Ｐ均＜ ０．００５），且与临床分期无关，民族与汉族之间的差异均无统计学意义（Ｐ＞ ０．０５）。（２）ＰＣＮＡ的阳性率在喉鳞状上皮细胞癌组织中的５７．８％ （４８／８３）高于喉乳头状瘤的３３．３％（２１／６３）和声带息肉的０．０％ （０／２０）（Ｐ＜ ０．００５），并与临床分期有关，Ⅳ期明显高于Ⅰ期（Ｐ＜ ０．０５），民、汉族之间无明显差异（Ｐ＞ ０．０５）。（３）经半定量分析，喉鳞状上皮细胞癌组织中的ｐ５３蛋白及ＰＣＮＡ 表达的阳性细胞密度及染色强度，明显高于喉乳头状瘤和声带息肉（Ｐ＜ ０．０５）。结论：（１）ｐ５３基因突变使ｐ５
Objective: To investigate the relationship between the expression of p53 protein and PCNA and the clinicopathological significance in the pathogenesis and progression of laryngeal papilloma and laryngeal squamous cell carcinoma in Xinjiang and Han nationalities. Methods: Thirty-six cases of laryngeal papilloma (33 cases of Uigur and 30 cases of Han), 83 cases of laryngeal squamous cell carcinoma (27 cases of Uygur, 4 cases of Kazak and 52 cases of Han) were studied by immunohistochemistry. Cases of vocal cord polyps (Uygur 10 cases, Han 10 cases) were tested. Results: (1) The positive rate of mutant p53 protein in laryngeal squamous cell carcinoma was 62.7% (52/83), which was significantly higher than that in laryngeal papilloma (28.5%, 24/63) and vocal cord polyp (P <0.05). There was no significant difference between ethnic groups and Han nationality (P> 0.05). (2) The positive rate of PCNA was 57.8% (48/83) in laryngeal squamous cell carcinoma than that in laryngeal papilloma 33.3% (21/63) and vocal polyp 0.0% (0/20) (P <0.005), which was related to clinical stage, stage Ⅳ was significantly higher than stage Ⅰ (P <0.05), there was no significant difference between people and Han (P> 0.05). (3) By semi-quantitative analysis, the positive cell density and staining intensity of p53 protein and PCNA expression in laryngeal squamous cell carcinoma were significantly higher than those in laryngeal papilloma and vocal cord polyps (P <0.05). Conclusion: (1) p53 gene mutation makes p5