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近几年,我国前列腺癌的发病率和致死率均明显升高。虽然早期肿瘤对去雄激素疗法敏感,但最终几乎所有病人均可转变为雄激素非依赖型。目前,对于此类病人还没有好的治疗手段和药物。11-脱氧轮枝菌素A(11’-deoxyverticillin A,C42)是一种从冬虫夏草共生菌中分离得到的多硫代二氧基哌嗪(Epipolythiodioxopiperazines,ETPs)族结构化合物,通过利用研究前列腺癌雄激素非依赖性癌细胞生长的常用细胞系PC3M细胞,就其对雄激素非依赖性前列腺癌细胞的凋亡及凋亡机制进行了研究。结果发现,C42能够显著抑制细胞生长,并增加caspase-3/7的活性及PARP的剪切。C42引起的这种caspase依赖的细胞凋亡与处理时间和该化合物的浓度相关。上述结果表明,C42能够诱导caspase依赖的细胞凋亡。进一步深入研究此类化合物将有助于理解其诱导程序性细胞死亡的机理,为开发此类化合物进行可能的临床治疗雄激素非依赖性前列腺癌打下了一定的理论基础。
In recent years, the incidence and mortality of prostate cancer in our country have obviously increased. Although early tumors are sensitive to androgen therapy, in the end almost all patients can switch to androgen-independent. At present, there is no good treatment and medicine for such patients. 11-deoxyverticillin A (C42) is an epipolythiodioxopiperazines (ETPs) family of structural compounds isolated from Cordyceps symbiosis bacteria, by using the study of prostate cancer Apoptosis and apoptosis mechanism of androgen-independent prostate cancer cells were studied in PC3M cells, a commonly used cell line that stimulates androgen-independent growth of cancer cells. The results showed that C42 can significantly inhibit cell growth and increase caspase-3/7 activity and PARP cleavage. This caspase-dependent apoptosis caused by C42 is related to the duration of treatment and the concentration of the compound. The above results indicate that C42 can induce caspase-dependent apoptosis. Further in-depth study of these compounds will help understand its mechanism of induction of programmed cell death, and lay a theoretical foundation for the development of such compounds for possible clinical treatment of androgen-independent prostate cancer.