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近年来砷对中枢神经系统的毒性作用逐渐受到人们的关注。本研究分析了砷暴露对大鼠原代AST分泌胶质细胞源性递质的影响。1资料与方法1.1实验动物与分组选3 d龄Wistar大鼠,将培养的AST分为0、2.5、5.0、10.0μmol/L砷暴露组,培养24 h后进行γ-氨基丁酸、D-丝氨酸、谷氨酸、甘氨酸、钙离子浓度指标的检测。1.2主要试剂胰蛋白酶;亚砷酸钠;fura2-AM染液;含有胎牛血清的DMEM培养基;无血清DMEM培
In recent years, the toxic effects of arsenic on the central nervous system have gradually drawn people’s attention. This study analyzed the effects of arsenic exposure on the primary neurotransmitter secretion of AST in rats. 1 Materials and Methods 1.1 Experimental animals and groups selected 3-day-old Wistar rats, the cultured AST were divided into 0, 2.5, 5.0, 10.0μmol / L arsenic exposure group, cultured for 24 h γ-aminobutyric acid, D- Serine, glutamic acid, glycine, calcium ion concentration test. 1.2 The main reagents trypsin; sodium arsenite; fura2-AM dye; DMEM medium containing fetal bovine serum; serum-free DMEM culture