目的　观察L 选择蛋白是否介导小鼠肝癌HCa F细胞与淋巴结黏附 ,探讨HCa F细胞特异性向周围淋巴结转移的分子机制。　方法　应用免疫细胞化学方法验证小鼠肝癌HCa F细胞为非淋巴细胞来源 ;用免疫印迹分析、RT PCR及流式细胞术检测L 选择蛋白在HCa F细胞的表达 ;并用L 选择蛋白的抗体抑制HCa F细胞与冰冻淋巴结切片间的黏附实验 (Stamper Woodruffmethod)检测L 选择蛋白的功能。　结果　HCa F细胞为非淋巴细胞来源的肝癌细胞 ,在其表面有L 选择蛋白的表达 ,且HCa F细胞与淋巴结之间的黏附可被L 选择蛋白的抗体抑制。结论　非淋巴细胞来源的HCa F细胞表面表达功能性L 选择蛋白分子 ,并可协助其与淋巴结黏附 ,从而可能介导HCa F细胞向淋巴道转移。 Objective To investigate whether L-selectin can mediate the adhesion of HCa F cells to lymph nodes in mouse hepatocellular carcinoma and to explore the molecular mechanism of HCa F cell-specific lymph node metastasis. Methods Immunocytochemistry was used to verify the origin of HCa F cells in mouse hepatoma. The expression of L-selectin in HCa F cells was detected by Western blot, RT PCR and flow cytometry. Antibodies against HCa F-cells and frozen lymph node sections between the adhesion test (Stamper Woodruffmethod) detection of L-selectin function. Results HCa F cells were non-lymphocyte-derived hepatocarcinoma cells with L-selectin expression on their surface, and the adhesion between HCa F cells and lymph nodes was inhibited by L-selectin antibody. Conclusion HCa F cells derived from non-lymphoid cells express functional L-selectin molecules on the surface of lymphocytes and can help them adhere to the lymph nodes, which may mediate the migration of HCa F cells into lymph nodes.