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为研究HBV诱发肝病的分子机制,建立了含2个HBV全基因组DNA的复制型转基因小鼠模型。经反复鉴定筛选,最后保留了3个品系(17、21、25)以供分析研究。在传代过程中(G_0代到G_4代),外源基因是按孟德尔规律稳定遗传的,并在G_2代时获得了外源基因纯合子的个体。同时在传代过程中,HBV DNA在血清中一直都稳定地出现。整合阳性小鼠的血清中能测出HBV DNA的比例高达94％(102/108),因此先测血清中HBV DNA的存在,反过来可对转基因小鼠作出可靠的筛选。收集92只G_2、G_3代整合阳性小鼠(12～20周龄)血清45ml,免疫负染处理
In order to study the molecular mechanism of HBV-induced liver disease, a replicative transgenic mouse model containing two HBV genomic DNAs was established. After repeated identification screening, the last retained three lines (17,21,25) for analysis. During the passage (G0 to G4), the exogenous gene was inherited according to Mendelian law and individuals homozygous for the exogenous gene were obtained at the G_2 generation. At the same time in the passage of HBV DNA in the serum has been stable. The percentage of HBV DNA that can be detected in the sera from positive, positive mice was as high as 94% (102/108), so serum HBV DNA was first detected and, in turn, a reliable screen for transgenic mice was made. Ninety-two G 2 and G 3 generation positive mice (12-20 weeks old) were collected for serum immunization