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目的探讨20(R)-人参皂甙Rg3(SPG-Rg3)对人乳腺癌MCF-7细胞的诱导凋亡作用及其可能机制。方法人乳腺癌细胞MCF-7细胞株分为空白对照组、实验对照组及SPG-Rg3多个浓度组。利用MTT法观察人参皂甙Rg3对MCF-7细胞生长的抑制作用,并计算出IC50,进一步确定其有效浓度;流式细胞术检测人参皂甙Rg3作用后MCF-7细胞周期的变化;利用PI单染法,检测人参皂甙Rg3诱导MCF-7细胞凋亡情况;免疫细胞化学染色检测MCF-7细胞凋亡和Fas和FasL蛋白的表达情况的关系。结果实验对照组和空白对照组间差别无统计学意义,其余各组间差别均有统计学意义。SPG-Rg3作用48h的IC50为244.54mg/L。流式细胞仪检测Rg3使MCF-7的S期细胞比率明显增加(P<0.01),凋亡率明显增加(P<0.01)。免疫细胞化学显示Rg3能使MCF-7的Fas和FasL蛋白表达增加(P<0.01)。结论 SPG-Rg3能诱导乳腺癌MCF-7细胞凋亡,其机制可能与上调Fas和FasL蛋白表达情况有关。
Objective To investigate the apoptosis-inducing effect of 20 (R) -ginsenoside Rg3 (SPG-Rg3) on human breast cancer MCF-7 cells and its possible mechanism. Methods Human breast cancer cell line MCF-7 was divided into blank control group, experimental control group and SPG-Rg3 multi-concentration group. The inhibitory effect of ginsenoside Rg3 on the growth of MCF-7 cells was observed by MTT assay, and the IC50 was calculated to determine its effective concentration. The changes of cell cycle of MCF-7 cells treated with ginsenoside Rg3 were detected by flow cytometry. Method to detect the apoptosis of MCF-7 cells induced by ginsenoside Rg3. The relationship between the apoptosis of MCF-7 cells and the expression of Fas and FasL proteins was detected by immunocytochemical staining. Results There was no significant difference between the experimental control group and the blank control group, and the differences among the other groups were statistically significant. The IC50 of SPG-Rg3 for 48h was 244.54mg / L. Flow cytometry detected Rg3 MCF-7 S phase cell ratio was significantly increased (P <0.01), the apoptosis rate was significantly increased (P <0.01). Immunocytochemistry showed that Rg3 increased the expression of Fas and FasL protein in MCF-7 (P <0.01). Conclusion SPG-Rg3 can induce apoptosis of breast cancer MCF-7 cells, and its mechanism may be related to the up-regulation of Fas and FasL protein expression.