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利用同源克隆法从小麦抗条锈病基因Yr5近等基因系(Taichung29*6/Yr5)克隆到编码Rop蛋白基因的全长cDNA序列,并将基因命名为TaRop3(Triticum aestivum Rop3),聚类分析其所在Rop蛋白家族中的亚组,并利用半定量RT-PCR方法分析其组织表达特异性和不同诱导条件下表达动态。序列分析表明,TaRop3开放阅读框为639 bp,预测编码含213个氨基酸残基Ⅱ型Rop蛋白,C末端具有膜定位基序,与大麦HvRop4和水稻OsRac4聚类在同一亚组。TaRop3基因在小麦幼苗和成株根、茎、叶片和茎节、柱头及花药中均有表达,其中在幼苗及成株茎部表达水平较高。非亲和条锈菌小种CYR17侵染和水杨酸处理诱导TaRop3表达增强,而干旱、高温胁迫以及脱落酸、乙烯利和茉莉酸甲酯处理均降低该基因表达水平。
The full-length cDNA sequence encoding the Rop gene was cloned from the Yr5 near isogenic line (Taichung29 * 6 / Yr5) of wheat stripe rust resistance by homologous cloning method. The gene was named TaRop3 (Triticum aestivum Rop3) Which belongs to the subgroup of Rop protein family, and analyzed its tissue expression specificity and expression under different induction conditions by semi-quantitative RT-PCR. Sequence analysis showed that the open reading frame of TaRop3 was 639 bp and predicted to encode a type II Rop protein with 213 amino acid residues. The Rop protein was located at the C terminus and was located in the same subgroup with HvRop4 and OsRac4. TaRop3 gene was expressed in wheat seedlings and adult roots, stems, leaves and stems, stigma and anthers, and the expression level of TaRop3 was higher in young plants and adult plants. Infection with CYR17 and salicylic acid could increase the expression of TaRop3, while drought, high temperature stress, abscisic acid, ethephon and methyl jasmonate all decreased the expression of TaRop3.