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目的 对贵州省鼠疫流动监测点采集的鼠类脏器标本即鼠肝脾的保存运输方法进行对比分析,为鼠肝脾的保存运输方法的改进提供依据,为鼠疫病原学检验质量控制提供科学依据.方法 将采集到的鼠肝脾标本采取常规保存液和甘油肉汤两种不同的方法进行保存运输,比较两种方法鼠肝脾培养结果及培养过程出现杂菌等情况的差异.结果 全年共使用常规保存液保存标本570份,培养48 h出现杂菌标本66份;使用甘油肉汤保存液保存标本570份,培养48 h出现杂菌41份;常规保存液与甘油肉汤保存液保存标本同时出现杂菌32份.全年累计标本数1 140份,均未检出鼠疫菌,实验过程中出现的疑似菌落鼠疫噬菌体实验结果均为阴性.全年7个县累计监测18次,有5次最后采样时间与送样时间间隔超过7d.随机抽样100份鼠脏器PCR检测结果均为阴性.结论 实验室检测结果质量控制中,使用无菌手段采集标本、冷链运输及运送时间需要得到保证.实验结果证实使用含有1/10万的龙胆紫甘油保存液冷冻保存鼠脏器标本可行.“,”Objective To identify and analysis the perserve approache in Guizhou Province,provide the basis for quality control of plague pathogen inspection.Methods Using two preservation solution is 570,the rat organs with glycerin preservation solution is 570.After different methods to storing and transporting in flow monitoring points in Guizhou Province.Parallel test and comparative test results.Results The rat organs with conventional training,all growth mixed bacteria is 32.Only growth mixed bacteria in conventional preservation solution is 34.Only growth mixed bacteria in glycerin preservation solution is 9.No plague bacteria.The PCR results are negative.Conclusion Experimental results confirm the feasibility of using 1/10 containing glycerol extender million crystalviolet cryopreserved rat organ specimens.