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以不同来源的柱状田头菇(Agrocybe aegerita)为研究对象,就不同的碳氮比、pH值以及培养温度对菌丝生长速度和结实性的影响进行了比较研究,并从同工酶角度分析比较了各菌株的亲缘关系。研究结果表明,不同来源的柱状田头菇所要求的最佳培养条件不尽相同。一般情况下,菌丝在碳氮比为10:1～80:1时均可生长,碳氮比为10:1时,菌丝生长最快,且易结实;柱状田头菇可生长并结实的pH值为4～12,最适宜结实的pH值为6;25℃时柱状田头菇菌丝生长速度最快,而20～25℃比较适合其结实。同工酶分析表明,柱状田头菇菌丝中过氧化物同工酶活性不高,酶带单调,不适宜用做检测遗传多样性的生化标记;酯酶同工酶有一定的活性,可以从相似系数上分析不同菌株的亲缘关系。
In this paper, Agrocybe aegerita from different sources was used to study the effects of different C / N ratio, pH value and culture temperature on mycelial growth rate and seed setting. The genetic relationship of each strain was compared. The results show that different sources of column Titian mushrooms required the best culture conditions vary. Under normal circumstances, mycelium growth in the C: N ratio of 10: 1 ~ 80: 1, C: N ratio of 10: 1, the mycelium grows the fastest, and easy to grow; column Agaricus can grow and strong The pH value is 4 ~ 12, the most suitable pH value is 6; Mycelia of column Agaricus bisporus grow fastest at 25 ℃, while 20 ~ 25 ℃ is more suitable for its strong. Isozyme analysis showed that the activity of peroxidase isozyme was not high and the enzyme band was monotonous, which was not suitable to be used as a biochemical marker to detect genetic diversity. Esterase isozyme had certain activity, Analyzing the genetic relationship of different strains from the similarity coefficient.